Isolation of ribosome stalling motifs from random libraries

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Although we tend to think that the ribosome can link together amino acids in any order, we have found that certain sequences interfere with protein synthesis. Characterization of stalling peptides found in bacteria has revealed multiple mechanisms of translational inhibition. Given that natural motifs differ in sequence and contain only three or four essential residues, peptide-mediated stalling may be quite widespread. To examine the scope of this phenomenon, we performed genetic selections in Escherichia coli to identify additional stalling motifs from random libraries. We characterized the mechanism of stalling with purified components using pre-steady-state kinetic methods. Some motifs block termination by inhibiting catalysis by release factors. In others, peptidyl transfer to certain aminoacyl-tRNAs is inhibited. Residues upstream of a stalling motif can either enhance or suppress these effects. One theme that emerges from these studies is the poor reactivity of proline, both as a peptidyl donor, at the C-terminus of the nascent peptide, and as a peptidyl acceptor, as prolyl-tRNA. This effect is compounded at three or more consecutive Pro codons. The translation factor EF-P alleviates stalling at polyproline sequences but has little or no effect on the motifs identified in our selections. Although our stalling motifs are in some sense artificial, several are underrepresented in bacterial proteomes, suggesting that they have been selected against, and analysis of ribosome profiling datasets reveals evidence of stalling where they occur in endogenous E. coli proteins.

Original languageEnglish (US)
Title of host publicationRegulatory Nascent Polypeptides
PublisherSpringer Japan
Pages225-240
Number of pages16
ISBN (Print)9784431550525, 4431550518, 9784431550518
DOIs
StatePublished - Apr 1 2014
Externally publishedYes

Fingerprint

Ribosomes
Libraries
Transfer RNA
Peptides
Genetic Selection
Escherichia coli Proteins
Proteome
Catalysis
Proline
Codon
Escherichia coli
Bacteria
Amino Acids
Kinetics
Proteins

Keywords

  • Bacteria
  • EF-P
  • Kinetics
  • Proline
  • RF1
  • Ribosome
  • Stalling
  • tmRNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Isolation of ribosome stalling motifs from random libraries. / Woolstenhulme, Christopher; Buskirk, Allen.

Regulatory Nascent Polypeptides. Springer Japan, 2014. p. 225-240.

Research output: Chapter in Book/Report/Conference proceedingChapter

Woolstenhulme, Christopher ; Buskirk, Allen. / Isolation of ribosome stalling motifs from random libraries. Regulatory Nascent Polypeptides. Springer Japan, 2014. pp. 225-240
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