This unit describes a method for isolation of plasma membrane sheets from rat liver. It also includes protocols for preparation of plasma membrane domains isolated from plasma membrane sheets and indirect immunofluorescence localization of marker proteins associated with plasma membrane sheets. The unit has been updated with assays for the marker enzymes alkaline phosphodiesterase I, 5' nucleotidase, and K+-stimulated.
|Original language||English (US)|
|Pages (from-to)||Unit 3.2|
|Journal||Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]|
|State||Published - May 2001|
ASJC Scopus subject areas
- Cell Biology