Isolation of proteins by heat-induced extraction from formalin-fixed, paraffin-embedded tissue and preparation of tryptic peptides for mass spectrometric analysis

Yuan Tian, Hui Zhang

Research output: Contribution to journalReview article

Abstract

This unit describes a method of isolating of proteins from formalin-fixed and paraffin-embedded (FFPE) tissue for mass spectrometry analysis. Heat-induced antigen retrieval is the basis of the protein extraction strategy presented in this protocol. This protocol may be used to identify nuclear, cytosolic, and membrane proteins from FFPE tissues extracted from tissue blocks or slides. Curr. Protoc. Mol. Biol. 90:10.26.1-10.26.7.

Original languageEnglish (US)
Pages (from-to)10.26.1-10.26.7
JournalCurrent protocols in molecular biology
Issue numberSUPPL. 90
DOIs
StatePublished - Apr 1 2010

Keywords

  • Formalin-fixed and paraffin-embedded tissue
  • Mass spectrometry
  • Peptide extraction
  • Protein identification

ASJC Scopus subject areas

  • Molecular Biology

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