Isolation of proteins by heat-induced extraction from formalin-fixed, paraffin-embedded tissue and preparation of tryptic peptides for mass spectrometric analysis

Yuan Tian; Hui Zhang

doi:10.1002/0471142727.mb1026s90

Isolation of proteins by heat-induced extraction from formalin-fixed, paraffin-embedded tissue and preparation of tryptic peptides for mass spectrometric analysis

Yuan Tian, Hui Zhang

School of Medicine

Research output: Contribution to journal › Review article › peer-review

4 Scopus citations

Abstract

This unit describes a method of isolating of proteins from formalin-fixed and paraffin-embedded (FFPE) tissue for mass spectrometry analysis. Heat-induced antigen retrieval is the basis of the protein extraction strategy presented in this protocol. This protocol may be used to identify nuclear, cytosolic, and membrane proteins from FFPE tissues extracted from tissue blocks or slides. Curr. Protoc. Mol. Biol. 90:10.26.1-10.26.7.

Original language	English (US)
Pages (from-to)	10.26.1-10.26.7
Journal	Current protocols in molecular biology
Issue number	SUPPL. 90
DOIs	https://doi.org/10.1002/0471142727.mb1026s90
State	Published - Apr 1 2010

Keywords

Formalin-fixed and paraffin-embedded tissue
Mass spectrometry
Peptide extraction
Protein identification

ASJC Scopus subject areas

Molecular Biology

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10.1002/0471142727.mb1026s90

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abstract = "This unit describes a method of isolating of proteins from formalin-fixed and paraffin-embedded (FFPE) tissue for mass spectrometry analysis. Heat-induced antigen retrieval is the basis of the protein extraction strategy presented in this protocol. This protocol may be used to identify nuclear, cytosolic, and membrane proteins from FFPE tissues extracted from tissue blocks or slides. Curr. Protoc. Mol. Biol. 90:10.26.1-10.26.7.",

keywords = "Formalin-fixed and paraffin-embedded tissue, Mass spectrometry, Peptide extraction, Protein identification",

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AU - Tian, Yuan

AU - Zhang, Hui

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Y1 - 2010/4/1

N2 - This unit describes a method of isolating of proteins from formalin-fixed and paraffin-embedded (FFPE) tissue for mass spectrometry analysis. Heat-induced antigen retrieval is the basis of the protein extraction strategy presented in this protocol. This protocol may be used to identify nuclear, cytosolic, and membrane proteins from FFPE tissues extracted from tissue blocks or slides. Curr. Protoc. Mol. Biol. 90:10.26.1-10.26.7.

AB - This unit describes a method of isolating of proteins from formalin-fixed and paraffin-embedded (FFPE) tissue for mass spectrometry analysis. Heat-induced antigen retrieval is the basis of the protein extraction strategy presented in this protocol. This protocol may be used to identify nuclear, cytosolic, and membrane proteins from FFPE tissues extracted from tissue blocks or slides. Curr. Protoc. Mol. Biol. 90:10.26.1-10.26.7.

KW - Formalin-fixed and paraffin-embedded tissue

KW - Mass spectrometry

KW - Peptide extraction

KW - Protein identification

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SP - 10.26.1-10.26.7

JO - Current Protocols in Molecular Biology

JF - Current Protocols in Molecular Biology

IS - SUPPL. 90

ER -

Isolation of proteins by heat-induced extraction from formalin-fixed, paraffin-embedded tissue and preparation of tryptic peptides for mass spectrometric analysis

Abstract

Keywords

ASJC Scopus subject areas

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