Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme

David S. Bredt, Solomon H. Snyder

Research output: Contribution to journalArticle

Abstract

Nitric oxide mediates vascular relaxing effects of endothelial cells, cytotoxic actions of macrophages and neutrophils, and influences of excitatory amino acids on cerebellar cyclic GMP. Its enzymatic formation from arginine by a soluble enzyme associated with stoichiometric production of citrulline requires NADPH and Ca2+. We show that nitric oxide synthetase activity requires calmodulin. Utilizing a 2′,5′-ADP affinity column eluted with NADPH, we have purified nitric oxide synthetase 6000-fold to homogeneity from rat cerebellum. The purified enzyme migrates as a single 150-kDa band on SDS/PAGE, and the native enzyme appears to be a monomer.

Original languageEnglish (US)
Pages (from-to)682-685
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number2
DOIs
StatePublished - Jan 1 1990

Keywords

  • Arginine
  • Endothelium-derived relaxing factor
  • cGMP

ASJC Scopus subject areas

  • General

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