Isolation of monochromosomal hybrids following fusion of human diploid fibroblast-derived microcells with mouse A9 cells

Yi Ning, M. Lovell, L. Taylor, O. M. Pereira-Smith

Research output: Contribution to journalArticle

Abstract

Rodent x human hybrids containing single hitman chromosomes are valuable tools in the study of human genetics. Using a retrovirus carrying the neomycin gene, we generated a neomycin-resistant (ncor) pool of human diploid fibroblasts. Following fusion of microcells prepared from this pool to mouse A9 cells, we obtained four ncor hybrids containing a single human chromosome 2, 3, 5, or 15.

Original languageEnglish (US)
Pages (from-to)79-80
Number of pages2
JournalCytogenetic and Genome Research
Volume60
Issue number1
DOIs
Publication statusPublished - 1992
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)
  • Cell Biology

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