Isolation of less polar alkali-labile glycolipids of human brain by high-speed countercurrent chromatography

Kazuhiro Matsuda, Ying Ma, Victoria Barghout, Yoichiro Ito, Subroto B Chatterjee

Research output: Contribution to journalArticle

Abstract

High-speed countercurrent chromatography (HSCCC), a liquid-liquid partition system, was used for the final purification of less polar alkali- labile glycolipids (ALGLs) of human brain. It has been reported that vertebrate brain contains ALGLs consisting of ester cerebroside and monoglucosyldiacylglycerol. ALGLs have alkali-labile ester bonds and are shown to be less polar than cerebroside. First, ALGLs (ALGL-I, II, III and IV) were extracted and isolated by repeated silica gel column chromatography. Then, the mixture of ALGLs was subjected to the HSCCC in which a solvent system, hexane/ethanol/water (5:4:1, by volume), was used with the lower phase mobile. ALGL-IV and -III were clearly separated. ALGL-IV, which was resolved as a single band on high performance thin-layer chromatography, was further separated into several components (ALGL-IVa, b, c, d and e). This is the first application of HSCCC for the separation of human brain ALGLs. The availability of purified ALGLs provides an opportunity to determine their structure, metabolic pathway, and function in relation to human health and disease.

Original languageEnglish (US)
Pages (from-to)103-110
Number of pages8
JournalJournal of Liquid Chromatography and Related Technologies
Volume21
Issue number1-2
StatePublished - 1998

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Countercurrent Distribution
Glycolipids
Alkalies
Chromatography
Brain
Cerebrosides
Esters
Thin layer chromatography
Column chromatography
Silica Gel
Liquids
Hexanes
Thin Layer Chromatography
Metabolic Networks and Pathways
Purification
Gel Chromatography
Vertebrates

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Analytical Chemistry

Cite this

Isolation of less polar alkali-labile glycolipids of human brain by high-speed countercurrent chromatography. / Matsuda, Kazuhiro; Ma, Ying; Barghout, Victoria; Ito, Yoichiro; Chatterjee, Subroto B.

In: Journal of Liquid Chromatography and Related Technologies, Vol. 21, No. 1-2, 1998, p. 103-110.

Research output: Contribution to journalArticle

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