Isolation of Japanese encephalitis virus from clinical specimens using a continuous mosquito cell line

C. J. Leake, D. S. Burke, A. Nisalak, C. H. Hoke

Research output: Contribution to journalArticlepeer-review

Abstract

During the 1983 Japanese encephalitis (JE) epidemic in northern Thailand, we systematically attempted to isolate JE virus (JEV) from clinical specimens collected from 49 consecutive JE patients at 1 provincial hospital. Fresh acute plasma and cerebrospinal fluid (CSF) samples and postmortem brain samples were immediately inoculated onto cultured monolayers of Aedes pseudoscutellaris (LSTM-AP-61) cells which had been shipped to the epidemic site. None of 49 plasma samples yielded virus. None of 30 fresh CSF samples from nonfatal cases yielded virus, but 5 of 15 (33%) CSF samples from fatal cases did. Inoculation of fresh brain specimens obtained at autopsy yielded virus in every case attempted (7 of 7), whereas postmortem needle biopsy specimens of brain yielded virus in only 1 of 4 cases. Isolates were most frequently successful using thalamic tissue (6 of 7 cases), but isolates were also commonly obtained from frontal cortex (4/7), occipital cortex (4/7), cerebellum (4/7), medulla (4/7) and pons (2/7).

Original languageEnglish (US)
Pages (from-to)1045-1050
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume35
Issue number5
DOIs
StatePublished - Jan 1 1986

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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