Isolation of human neuronal cells resistant to toxicity by the prion protein peptide 106-126

Y. Gu, Y. Jing, A. Kumar, Y. Sharma, H. Fujioka, N. Singh

Research output: Contribution to journalArticle

Abstract

Prion diseases or transmissible spongiform encephalopathies, are neurodegenerative disorders that are genetic, sporadic, or infectious. The pathogenetic event common to all prion disorders is the conformational transformation of the cellular prion protein (PrPC) to the scrapie form (PrPSc), that deposits in the brain parenchyma and induces neuronal death. Infectious prion disorders are caused by exogenously introduced PrPSc that acts as a template in the conversion of endogenous PrPC to nascent PrPSc, and subsequently the process becomes autocatalytic. To understand the process of cellular uptake of PrPSc and its mechanism of cellular toxicity, previous studies have used a PrP fragment spanning residues 106-126 (PrPTx) that is toxic to primary neurons in culture, and mimics PrPSc in its biophysical properties [9,11,14]. Several possible mechanisms of cell death by PrPTx have been proposed [2,3,10,11,18], but the existing data are unclear. To identify the biochemical pathways of neurotoxicity by this fragment, we have isolated mutant neuroblastoma and NT-2 cells that are resistant to toxicity by PrPTx. We show that these cells bind and internalize PrPTx in a temperature dependent fashion, and the peptide accumulates in intracellular compartments, probably lysosomes, where it has an unusually long half-life. The PrPTx-resistant phenotype of the cells reported in this study could result from aberrant binding or internalization of the peptide, or due to an abnormality in the downstream pathway(s) of neuronal toxicity. The PrPTx-resistant cells are therefore a useful tool for evaluating the cellular and biochemical pathways that lead to cell death by this peptide, and will provide insight into the mechanism(s) of neurotoxicity by PrPSc.

Original languageEnglish (US)
Pages (from-to)169-180
Number of pages12
JournalJournal of Alzheimer's Disease
Volume3
Issue number2
DOIs
StatePublished - Jan 1 2001

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Keywords

  • Prion
  • Resistant cells
  • Toxic peptide

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Psychology
  • Geriatrics and Gerontology
  • Psychiatry and Mental health

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