Isolation of human basophils

John T. Schroeder; Anja P. Bieneman

doi:10.1002/0471142735.im0724s112

Isolation of human basophils

John T. Schroeder, Anja P. Bieneman

School of Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Isolating human basophils from blood has long been hampered by the fact that these granulocytes represent just 1% or less of the circulating leukocyte population.We describe herein laboratory protocols that have been refined over the past ~25 years that now enable investigators to prepare basophils for use in a variety of assays to assess the in vitro biology of these immune cells, both in IgE -dependent and -independent responses.

Original language	English (US)
Pages (from-to)	7.24.1-7.24.8
Journal	Current protocols in immunology
Volume	2016
DOIs	https://doi.org/10.1002/0471142735.im0724s112
State	Published - 2016

Keywords

Immune disease
Immunologic studies in humans
In vitro assays for immune cell function
Innate immunity

ASJC Scopus subject areas

Immunology

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10.1002/0471142735.im0724s112

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title = "Isolation of human basophils",

abstract = "Isolating human basophils from blood has long been hampered by the fact that these granulocytes represent just 1% or less of the circulating leukocyte population.We describe herein laboratory protocols that have been refined over the past ~25 years that now enable investigators to prepare basophils for use in a variety of assays to assess the in vitro biology of these immune cells, both in IgE -dependent and -independent responses.",

keywords = "Immune disease, Immunologic studies in humans, In vitro assays for immune cell function, Innate immunity",

author = "Schroeder, {John T.} and Bieneman, {Anja P.}",

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AU - Bieneman, Anja P.

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AB - Isolating human basophils from blood has long been hampered by the fact that these granulocytes represent just 1% or less of the circulating leukocyte population.We describe herein laboratory protocols that have been refined over the past ~25 years that now enable investigators to prepare basophils for use in a variety of assays to assess the in vitro biology of these immune cells, both in IgE -dependent and -independent responses.

KW - Immune disease

KW - Immunologic studies in humans

KW - In vitro assays for immune cell function

KW - Innate immunity

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ER -

Isolation of human basophils

Abstract

Keywords

ASJC Scopus subject areas

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