Isolation of Human Basophils

John Thomas Schroeder, Anja P. Bieneman

Research output: Contribution to journalArticle

Abstract

Isolating human basophils from blood has long been hampered by the fact that these granulocytes represent just 1% or less of the circulating leukocyte population. We describe herein laboratory protocols that have been refined over the past ∼25 years that now enable investigators to prepare basophils for use in a variety of assays to assess the in vitro biology of these immune cells, both in IgE -dependent and -independent responses.

Original languageEnglish (US)
JournalCurrent Protocols in Immunology
Volume112
DOIs
StatePublished - Feb 2 2016

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Basophils
Granulocytes
Immunoglobulin E
Cell Biology
Leukocytes
Research Personnel
Population
In Vitro Techniques

Keywords

  • immune disease
  • immunologic studies in humans
  • in vitro assays for immune cell function
  • innate immunity

ASJC Scopus subject areas

  • Immunology

Cite this

Isolation of Human Basophils. / Schroeder, John Thomas; Bieneman, Anja P.

In: Current Protocols in Immunology, Vol. 112, 02.02.2016.

Research output: Contribution to journalArticle

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