Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida

Pierre E. Rollin; Thomas G. Ksiazek; Luanne H. Elliott; Eugene V. Ravkov; Mary Lane Martin; Sergey Morzunov; Walter Livingstone; Marty Monroe; Gregory Glass; Sup Ruo; Ali S. Khan; James E. Childs; Stuart T. Nichol; C. J. Peters

doi:10.1002/jmv.1890460108

Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida

Pierre E. Rollin, Thomas G. Ksiazek, Luanne H. Elliott, Eugene V. Ravkov, Mary Lane Martin, Sergey Morzunov, Walter Livingstone, Marty Monroe, Gregory Glass, Sup Ruo, Ali S. Khan, James E. Childs, Stuart T. Nichol, C. J. Peters

Research output: Contribution to journal › Article › peer-review

126 Scopus citations

Abstract

Numerous rodents were trapped for serologic and virologic studies following the identification of a hantavirus pulmonary syndrome (HPS) case in Dade County, Florida. Cotton rats (Sigmodon hispidus) were the most frequently captured rodent and displayed the highest seroprevalence to a variety of hantavirus antigens. Hantavirus genome RNA was detected in all the seropositive cotton rats tested, using a reverse transcriptase‐polymerase chain reaction (RT‐PCR) assay. A virus was isolated from tissues of two seropositive cotton rats by cultivation of lung and spleen homogenates on Vero E6 cells. Nucleotide sequence information obtained by direct RT‐PCR and the serologic relationships of this virus with the other hantaviruses indicate that this virus, Black Creek Canal virus, represents a new hantavirus distinct from the previously known serotypes. © 1995 Wiley‐Liss, Inc.

Original language	English (US)
Pages (from-to)	35-39
Number of pages	5
Journal	Journal of Medical Virology
Volume	46
Issue number	1
DOIs	https://doi.org/10.1002/jmv.1890460108
State	Published - May 1995
Externally published	Yes

Keywords

Bunyaviridae
hantavirus pulmonary syndrome
rodent

ASJC Scopus subject areas

Virology
Infectious Diseases

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10.1002/jmv.1890460108

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Rollin, P. E., Ksiazek, T. G., Elliott, L. H., Ravkov, E. V., Martin, M. L., Morzunov, S., Livingstone, W., Monroe, M., Glass, G., Ruo, S., Khan, A. S., Childs, J. E., Nichol, S. T., & Peters, C. J. (1995). Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida. Journal of Medical Virology, 46(1), 35-39. https://doi.org/10.1002/jmv.1890460108

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abstract = "Numerous rodents were trapped for serologic and virologic studies following the identification of a hantavirus pulmonary syndrome (HPS) case in Dade County, Florida. Cotton rats (Sigmodon hispidus) were the most frequently captured rodent and displayed the highest seroprevalence to a variety of hantavirus antigens. Hantavirus genome RNA was detected in all the seropositive cotton rats tested, using a reverse transcriptase‐polymerase chain reaction (RT‐PCR) assay. A virus was isolated from tissues of two seropositive cotton rats by cultivation of lung and spleen homogenates on Vero E6 cells. Nucleotide sequence information obtained by direct RT‐PCR and the serologic relationships of this virus with the other hantaviruses indicate that this virus, Black Creek Canal virus, represents a new hantavirus distinct from the previously known serotypes. {\textcopyright} 1995 Wiley‐Liss, Inc.",

keywords = "Bunyaviridae, hantavirus pulmonary syndrome, rodent",

author = "Rollin, {Pierre E.} and Ksiazek, {Thomas G.} and Elliott, {Luanne H.} and Ravkov, {Eugene V.} and Martin, {Mary Lane} and Sergey Morzunov and Walter Livingstone and Marty Monroe and Gregory Glass and Sup Ruo and Khan, {Ali S.} and Childs, {James E.} and Nichol, {Stuart T.} and Peters, {C. J.}",

year = "1995",

month = may,

doi = "10.1002/jmv.1890460108",

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AU - Ravkov, Eugene V.

AU - Martin, Mary Lane

AU - Morzunov, Sergey

AU - Livingstone, Walter

AU - Monroe, Marty

AU - Glass, Gregory

AU - Ruo, Sup

AU - Khan, Ali S.

AU - Childs, James E.

AU - Nichol, Stuart T.

AU - Peters, C. J.

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KW - Bunyaviridae

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Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida

Abstract

Keywords

ASJC Scopus subject areas

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