Isolation of an olfactory cDNA: Similarity to retinol-binding protein suggests a role in olfaction

Kyu Ho Lee, Rebecca G. Wells, Randall R Reed

Research output: Contribution to journalArticle

Abstract

Molecular loning techniques were used to isolate and characterize a protein possibly involved in the signal transducing system in olfactory tissue of the frog Rana pipiens. A complementary DNA library was constructed with messenger RNA obtained from frog olfactory neuroepithelium. A 700-base pair complementary DNA done encoding a protein with a molecular weight of 20,300 was identified by differential hybridization analysis with polyadenylated RNA from olfactory epithelium and nonsensory respiratory epithelium. The messenger RNA corresponding to this done was abundant in the cells of Bowman's glands in olfactory tissue but not in respiratory epithelium nor in several other tissues. The predicted sequence of this protein is homologous to members of a family of proteins that bind and transport small molecules in serum, suggesting that this protein may also bind and transport odorants in the mucus secreted by Bowman's glands.

Original languageEnglish (US)
Pages (from-to)1053-1056
Number of pages4
JournalScience
Volume235
Issue number4792
StatePublished - 1987

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Retinol-Binding Proteins
Smell
Respiratory Mucosa
Complementary DNA
Anura
Messenger RNA
Rana pipiens
Olfactory Mucosa
Proteins
Protein Transport
Mucus
Gene Library
Base Pairing
Blood Proteins
Molecular Weight

ASJC Scopus subject areas

  • General

Cite this

Isolation of an olfactory cDNA : Similarity to retinol-binding protein suggests a role in olfaction. / Lee, Kyu Ho; Wells, Rebecca G.; Reed, Randall R.

In: Science, Vol. 235, No. 4792, 1987, p. 1053-1056.

Research output: Contribution to journalArticle

Lee, Kyu Ho ; Wells, Rebecca G. ; Reed, Randall R. / Isolation of an olfactory cDNA : Similarity to retinol-binding protein suggests a role in olfaction. In: Science. 1987 ; Vol. 235, No. 4792. pp. 1053-1056.
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