Isolation of a marker linked to the Charcot-Marie-Tooth disease type IA gene by differential Alu-PCR of human chromosome 17-retaining hybrids

P. I. Patel, C. Garcia, R. Montes de Oca-Luna, R. I. Malamut, B. Franco, S. Slaugenhaupt, A. Chakravarti, J. R. Lupski

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Abstract

We report the isolation of a new marker (S6.1) from band p11.2 of human chromosome 17 by differential Alu-polymerase chain reaction (Alu-PCR) of both a monochromosomal hybrid retaining a single human chromosome 17 and a hybrid retaining a del(17)(p11.2p11.2) in addition to other human chromosomes. The method is based on the preferential PCR amplification of human DNA in rodent/human hybrids when primers specific to the human Alu repeat element are used. MspI and SstI RFLPs associated with S6.1 were identified and used in linkage analysis of both a previously reported and a newly identified French-Acadian kindred segregating autosomal dominant Charcot-Marie-Tooth disease (CMT). A cumulative peak lod score of 3.41 at a peak recombination fraction of .12 indicates that this marker is linked to the CMT 1A locus but is at a distance from the disease gene. Thus, the marker S6.1 will be useful in further delineating the candidate region for the CMT gene when its location with respect to pA10-41 and 1516, two other markers from 17p11.2 which have previously demonstrated close linkage to the CMT locus, has been determined.

Original languageEnglish (US)
Pages (from-to)926-934
Number of pages9
JournalAmerican journal of human genetics
Volume47
Issue number6
StatePublished - Dec 4 1990

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ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Patel, P. I., Garcia, C., Montes de Oca-Luna, R., Malamut, R. I., Franco, B., Slaugenhaupt, S., Chakravarti, A., & Lupski, J. R. (1990). Isolation of a marker linked to the Charcot-Marie-Tooth disease type IA gene by differential Alu-PCR of human chromosome 17-retaining hybrids. American journal of human genetics, 47(6), 926-934.