Isolation, growth, and purification of defective adenovirus deletion mutants.

Gary W Ketner, Julie Boyer

Research output: Contribution to journalArticle

Abstract

Defective adenovirus deletion mutants can be grown by complementation in the presence of helper viruses that supply essential functions missing in the deletion mutant. In general, the deletion mutant then must be separated physically from the helper for use in subsequent experiments. This chapter includes suggestions for selection of helper viruses, protocols for the production of stocks by complementation, and procedures for physical separation of deletion mutants from their helpers.

Original languageEnglish (US)
Pages (from-to)19-28
Number of pages10
JournalMethods in molecular medicine
Volume130
StatePublished - 2007

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Helper Viruses
Adenoviridae
Growth

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Isolation, growth, and purification of defective adenovirus deletion mutants. / Ketner, Gary W; Boyer, Julie.

In: Methods in molecular medicine, Vol. 130, 2007, p. 19-28.

Research output: Contribution to journalArticle

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