Isolation, growth, and purification of defective adenovirus deletion mutants.

Gary Ketner, Julie Boyer

Research output: Contribution to journalArticle

Abstract

Defective adenovirus deletion mutants can be grown by complementation in the presence of helper viruses that supply essential functions missing in the deletion mutant. In general, the deletion mutant then must be separated physically from the helper for use in subsequent experiments. This chapter includes suggestions for selection of helper viruses, protocols for the production of stocks by complementation, and procedures for physical separation of deletion mutants from their helpers.

Original languageEnglish (US)
Pages (from-to)19-28
Number of pages10
JournalMethods in Molecular Medicine
Volume130
StatePublished - 2007

ASJC Scopus subject areas

  • Molecular Medicine

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