Isolation and proteomic alalysis of cell wall-deficient Haematococcus pluvialis mutants

Sheng Bing Wang, Feng Chen, Milton Sommerfeld, Qiang Hu

Research output: Contribution to journalArticle

Abstract

The green alga Haematococcus pluvialis has a plant-like cell wall consisting of glycoproteins and cellulose that is modified during the cell cycle and under various conditions. These features allow Haematococcus to be used as a model organism for studying cell wall biology. Development of the Haematococcus model is hampered by the absence of mutants that could provide insight into the biosynthesis and assembly of wall components. Haematococcus mutants (WM#537 and WM#2978) (WM-wall mutant) with defective cell walls were obtained by chemical mutagenesis. WM#537 features a secondary wall of considerably reduced thickness, whereas WM#2978 possesses a somewhat reduced secondary wall with little intervening space between the wall and plasmalemma. 2-DE revealed that a majority of the cell wall proteins were present in the wild-type and mutant cell walls throughout the cell cycle. PMF identified 55 wall protein orthologs from these strains, including a subset of induced proteins known to be involved in wall construction, remodeling, and defense. Down-regulation of certain wall proteins in the two mutants was associated with the wall defects, whereas overexpression of other proteins may have compensated for the defective walls in the two mutants.

Original languageEnglish (US)
Pages (from-to)4839-4851
Number of pages13
JournalProteomics
Volume5
Issue number18
DOIs
StatePublished - Dec 1 2005

Keywords

  • Cell wall proteins
  • Cell wall-deficient mutant
  • Haematococcus pluvialis
  • Microalgae
  • PMF

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Isolation and proteomic alalysis of cell wall-deficient Haematococcus pluvialis mutants'. Together they form a unique fingerprint.

  • Cite this