Isolation and preliminary characterization of Escherichia coli mutants deficient in exonuclease VII

L. D. Vales, B. A. Rabin, J. W. Chase

Research output: Contribution to journalArticlepeer-review

Abstract

Strains of Escherichia coli containing reduced levels of exonuclease VII activity due to mutations in the xseB gene have been isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Seven mutants of independent origin deficient in exonuclease VII activity were obtained. Four of these contained defects in xseA, a locus which has been previously identified, and three others contained mutations in a gene distinct from xseA, which we have designated xseB. Genetic mapping studies place the xseB locus between proC and dnaZ. Exonuclease VII purified from KLC835 (xseA+ xseB3) is more heat labile than enzyme purified from the parent strain PA610 (xse+), showing that xseB is a structural gene for exonuclease VII. The isolation of λ transducing phage carrying xseA is also described.

Original languageEnglish (US)
Pages (from-to)1116-1122
Number of pages7
JournalJournal of bacteriology
Volume155
Issue number3
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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