Abstract
We have isolated two clones containing 19 kilobases (kb) of the human gene coding for a pro-αl(II) collagen chain from human λ genomic DNA libraries. A 3' clone, HC2A, was selected by cross-hybridization with a cDNA clone containing sequences coding for the carboxy propeptide of chick type II procollagen. A second clone, HC2B, was obtained by screening the library with the 5' part of HC2A. The sequence analysis of exon 3 corresponding to the C propeptide reveals the presence of stretches of conserved nucleotides between the human and the chick type II procollagen genes. On Northern blots, the human collagen clone hybridizes strongly to a 5.5-kb RNA for the rat type II procollagen chain. Finally, studies of genomic DNAs from normal individuals reveal the presence of a HindIII and a BamHl polymorphic site at the 3' end of the gene.
Original language | English (US) |
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Pages (from-to) | 6343-6348 |
Number of pages | 6 |
Journal | Biochemistry |
Volume | 24 |
Issue number | 23 |
DOIs | |
State | Published - Nov 1 1985 |
ASJC Scopus subject areas
- Biochemistry