Isolation and Partial Characterization of Genomic Clones Coding for a Human Pro-αl(II) Collagen Chain and Demonstration of Restriction Fragment Length Polymorphism at the 3' End of the Gene

Anne Marie Nunez, George R. Martin, Yoshihiko Yamada, Clair Francomano, Marian F. Young

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

We have isolated two clones containing 19 kilobases (kb) of the human gene coding for a pro-αl(II) collagen chain from human λ genomic DNA libraries. A 3' clone, HC2A, was selected by cross-hybridization with a cDNA clone containing sequences coding for the carboxy propeptide of chick type II procollagen. A second clone, HC2B, was obtained by screening the library with the 5' part of HC2A. The sequence analysis of exon 3 corresponding to the C propeptide reveals the presence of stretches of conserved nucleotides between the human and the chick type II procollagen genes. On Northern blots, the human collagen clone hybridizes strongly to a 5.5-kb RNA for the rat type II procollagen chain. Finally, studies of genomic DNAs from normal individuals reveal the presence of a HindIII and a BamHl polymorphic site at the 3' end of the gene.

Original languageEnglish (US)
Pages (from-to)6343-6348
Number of pages6
JournalBiochemistry
Volume24
Issue number23
DOIs
StatePublished - Nov 1 1985

ASJC Scopus subject areas

  • Biochemistry

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