Isolation and partial characterization of genomic clones coding for a human pro-α1(II) collagen chain and demonstration of restriction fragment length polymorphism at the 3′ end of the gene

Anne Marie Nunez, Clair Francomano, Marian F. Young, Clair Ann Francomano, Yoshihiko Yamada

Research output: Contribution to journalArticle

Abstract

We have isolated two clones containing 19 kilobases (kb) of the human gene coding for a pro-α1(II) collagen chain from human λ genomic DNA libraries. A 3′ clone, HC2A, was selected by cross-hybridization with a cDNA clone containing sequences coding for the carboxy propeptide of chick type II procollagen. A second clone, HC2B, was obtained by screening the library with the 5′ part of HC2A. The sequence analysis of exon 3 corresponding to the C propeptide reveals the presence of stretches of conserved nucleotides between the human and the chick type II procollagen genes. On Northern blots, the human collagen clone hybridizes strongly to a 5.5-kb RNA for the rat type II procollagen chain. Finally, studies of genomic DNAs from normal individuals reveal the presence of a HindIII and a BamHI polymorphic site at the 3′ end of the gene.

Original languageEnglish (US)
Pages (from-to)6343-6348
Number of pages6
JournalBiochemistry®
Volume24
Issue number23
StatePublished - 1985

Fingerprint

Collagen Type II
Polymorphism
Restriction Fragment Length Polymorphisms
Demonstrations
Collagen
Clone Cells
Genes
Gene Library
Rats
Exons
Screening
Nucleotides
Complementary DNA
Procollagen
RNA
Genomic Library
Northern Blotting
DNA
Libraries
Sequence Analysis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Isolation and partial characterization of genomic clones coding for a human pro-α1(II) collagen chain and demonstration of restriction fragment length polymorphism at the 3′ end of the gene. / Nunez, Anne Marie; Francomano, Clair; Young, Marian F.; Francomano, Clair Ann; Yamada, Yoshihiko.

In: Biochemistry®, Vol. 24, No. 23, 1985, p. 6343-6348.

Research output: Contribution to journalArticle

@article{0924e8f3e5434a678f78576568ab515c,
title = "Isolation and partial characterization of genomic clones coding for a human pro-α1(II) collagen chain and demonstration of restriction fragment length polymorphism at the 3′ end of the gene",
abstract = "We have isolated two clones containing 19 kilobases (kb) of the human gene coding for a pro-α1(II) collagen chain from human λ genomic DNA libraries. A 3′ clone, HC2A, was selected by cross-hybridization with a cDNA clone containing sequences coding for the carboxy propeptide of chick type II procollagen. A second clone, HC2B, was obtained by screening the library with the 5′ part of HC2A. The sequence analysis of exon 3 corresponding to the C propeptide reveals the presence of stretches of conserved nucleotides between the human and the chick type II procollagen genes. On Northern blots, the human collagen clone hybridizes strongly to a 5.5-kb RNA for the rat type II procollagen chain. Finally, studies of genomic DNAs from normal individuals reveal the presence of a HindIII and a BamHI polymorphic site at the 3′ end of the gene.",
author = "Nunez, {Anne Marie} and Clair Francomano and Young, {Marian F.} and Francomano, {Clair Ann} and Yoshihiko Yamada",
year = "1985",
language = "English (US)",
volume = "24",
pages = "6343--6348",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "23",

}

TY - JOUR

T1 - Isolation and partial characterization of genomic clones coding for a human pro-α1(II) collagen chain and demonstration of restriction fragment length polymorphism at the 3′ end of the gene

AU - Nunez, Anne Marie

AU - Francomano, Clair

AU - Young, Marian F.

AU - Francomano, Clair Ann

AU - Yamada, Yoshihiko

PY - 1985

Y1 - 1985

N2 - We have isolated two clones containing 19 kilobases (kb) of the human gene coding for a pro-α1(II) collagen chain from human λ genomic DNA libraries. A 3′ clone, HC2A, was selected by cross-hybridization with a cDNA clone containing sequences coding for the carboxy propeptide of chick type II procollagen. A second clone, HC2B, was obtained by screening the library with the 5′ part of HC2A. The sequence analysis of exon 3 corresponding to the C propeptide reveals the presence of stretches of conserved nucleotides between the human and the chick type II procollagen genes. On Northern blots, the human collagen clone hybridizes strongly to a 5.5-kb RNA for the rat type II procollagen chain. Finally, studies of genomic DNAs from normal individuals reveal the presence of a HindIII and a BamHI polymorphic site at the 3′ end of the gene.

AB - We have isolated two clones containing 19 kilobases (kb) of the human gene coding for a pro-α1(II) collagen chain from human λ genomic DNA libraries. A 3′ clone, HC2A, was selected by cross-hybridization with a cDNA clone containing sequences coding for the carboxy propeptide of chick type II procollagen. A second clone, HC2B, was obtained by screening the library with the 5′ part of HC2A. The sequence analysis of exon 3 corresponding to the C propeptide reveals the presence of stretches of conserved nucleotides between the human and the chick type II procollagen genes. On Northern blots, the human collagen clone hybridizes strongly to a 5.5-kb RNA for the rat type II procollagen chain. Finally, studies of genomic DNAs from normal individuals reveal the presence of a HindIII and a BamHI polymorphic site at the 3′ end of the gene.

UR - http://www.scopus.com/inward/record.url?scp=0022413496&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022413496&partnerID=8YFLogxK

M3 - Article

C2 - 3002437

AN - SCOPUS:0022413496

VL - 24

SP - 6343

EP - 6348

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 23

ER -