Isolation and mechanical measurements of myofibrils from human induced pluripotent stem cell-derived cardiomyocytes

Josè Manuel Pioner, Alice W. Racca, Jordan M. Klaiman, Kai Chun Yang, Xuan Guan, Lil Pabon, Veronica Muskheli, Rebecca Zaunbrecher, Jesse Macadangdang, Mark Y. Jeong, David L. Mack, Martin K. Childers, Deok Ho Kim, Chiara Tesi, Corrado Poggesi, Charles E. Murry, Michael Regnier

Research output: Contribution to journalArticlepeer-review

Abstract

Tension production and contractile properties are poorly characterized aspects of excitation-contraction coupling of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Previous approaches have been limited due to the small size and structural immaturity of early-stage hiPSC-CMs. We developed a substrate nanopatterning approach to produce hiPSC-CMs in culture with adult-like dimensions, T-tubule-like structures, and aligned myofibrils. We then isolated myofibrils from hiPSC-CMs and measured the tension and kinetics of activation and relaxation using a custom-built apparatus with fast solution switching. The contractile properties and ultrastructure of myofibrils more closely resembled human fetal myofibrils of similar gestational age than adult preparations. We also demonstrated the ability to study the development of contractile dysfunction of myofibrils from a patient-derived hiPSC-CM cell line carrying the familial cardiomyopathy MYH7 mutation (E848G). These methods can bring new insights to understanding cardiomyocyte maturation and developmental mechanical dysfunction of hiPSC-CMs with cardiomyopathic mutations.

Original languageEnglish (US)
Pages (from-to)885-896
Number of pages12
JournalStem Cell Reports
Volume6
Issue number6
DOIs
StatePublished - Jun 14 2016
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

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