Isolation and characterization of Tn7 transposase gain-of-function mutants: A model for transposase activation

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Abstract

Tn7 transposition has been hypothesized to require a heteromeric transposase formed by two Tn7-encoded proteins, TnsA and TnsB, and accessory proteins that activate the transposase when they are associated with an appropriate target DNA. This study investigates the mechanism of Tn7 transposase activation by isolation and analysis of transposase gain-of-function mutants that are active in the absence of these accessory proteins. This work shows directly that TnsA and TnsB are essential and sufficient components of the Tn7 transposase and also provides insight into the signals that activate the transposase. We also describe a protein-protein interaction between TnsA and TnsC, a regulatory accessory protein, that is likely to be critical for transposase activation.

Original languageEnglish (US)
Pages (from-to)3446-3457
Number of pages12
JournalEMBO Journal
Volume19
Issue number13
DOIs
StatePublished - Jul 3 2000

Keywords

  • ATP
  • Conformational change
  • Enzyme activation
  • Protein-protein interaction
  • Transposition

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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