Isolation and Characterization of Sea Star Factor

R. A. PRENDERGAST, S. H. LIU

Research output: Contribution to journalArticlepeer-review

Abstract

Sea star factor (SSF) harvested from the echinoderm Asterias forbesi celomocytes is a potent inhibitor of the primary immune response to T‐dependent antigens and also suppresses concanavalin‐A‐induced mitogenesis. We have isolated and purified a basic protein from this source which has a molecular weight of approximately 38,000 daltons and is responsible for these biological activities. After complete reduction, SSF was shown to be composed of a single pair of heavy and light chains. Preincubation of spleen cells with concanavalin A does not inhibit binding of SSF; however, specific binding can be inhibited by preincubation with unlabeled SSF. These data suggest that the binding site for SSF is not the same as that for concanavalin A.

Original languageEnglish (US)
Pages (from-to)873-880
Number of pages8
JournalScandinavian Journal of Immunology
Volume5
Issue number6-7
DOIs
StatePublished - Jun 1976

ASJC Scopus subject areas

  • Immunology

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