Isolation and characterization of protective cytolytic T cells in a rodent malaria model system

Pedro Romero, Janet L. Maryanski, Anne Sophie Cordey, Giampietro Corradin, Ruth S. Nussenzweig, Fidel P Zavala

Research output: Contribution to journalArticle

Abstract

Protective immunity against malaria is induced by immunization with irradiation-attenuated sporozoites. Here we report the isolation of cytolytic T-cell (CTL) clones from BALB/c (H-2d) mice immunized with either Plasmodium berghei or Plasmodium yoelii sporozoites. The epitopes recognized by these CTL can be mimicked by synthetic peptides corresponding to a homologous region in the CS proteins of both rodent malaria species. Both peptides are recognized by the CTL in the context of the same MHC class I molecule, H-2 Kd. In vivo adoptive transfer of the CTL clones into non-immune syngeneic mice protected them from a lethal challenge of infectious sporozoites.

Original languageEnglish (US)
Pages (from-to)27-31
Number of pages5
JournalImmunology Letters
Volume25
Issue number1-3
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Sporozoites
Malaria
Rodentia
T-Lymphocytes
Clone Cells
Plasmodium yoelii
Plasmodium berghei
Peptides
Adoptive Transfer
Epitopes
Immunity
Immunization
Proteins

Keywords

  • Circumsporozoite (CS) protein
  • Cytololytic T cells (CTL)
  • Malaria
  • Plasmodium berghei
  • Plasmodium yoelii

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Isolation and characterization of protective cytolytic T cells in a rodent malaria model system. / Romero, Pedro; Maryanski, Janet L.; Cordey, Anne Sophie; Corradin, Giampietro; Nussenzweig, Ruth S.; Zavala, Fidel P.

In: Immunology Letters, Vol. 25, No. 1-3, 1990, p. 27-31.

Research output: Contribution to journalArticle

Romero, Pedro ; Maryanski, Janet L. ; Cordey, Anne Sophie ; Corradin, Giampietro ; Nussenzweig, Ruth S. ; Zavala, Fidel P. / Isolation and characterization of protective cytolytic T cells in a rodent malaria model system. In: Immunology Letters. 1990 ; Vol. 25, No. 1-3. pp. 27-31.
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