Isolation and characterization of protective cytolytic T cells in a rodent malaria model system

Pedro Romero, Janet L. Maryanski, Anne Sophie Cordey, Giampietro Corradin, Ruth S. Nussenzweig, Fidel Zavala

Research output: Contribution to journalArticlepeer-review

Abstract

Protective immunity against malaria is induced by immunization with irradiation-attenuated sporozoites. Here we report the isolation of cytolytic T-cell (CTL) clones from BALB/c (H-2d) mice immunized with either Plasmodium berghei or Plasmodium yoelii sporozoites. The epitopes recognized by these CTL can be mimicked by synthetic peptides corresponding to a homologous region in the CS proteins of both rodent malaria species. Both peptides are recognized by the CTL in the context of the same MHC class I molecule, H-2 Kd. In vivo adoptive transfer of the CTL clones into non-immune syngeneic mice protected them from a lethal challenge of infectious sporozoites.

Original languageEnglish (US)
Pages (from-to)27-31
Number of pages5
JournalImmunology Letters
Volume25
Issue number1-3
DOIs
StatePublished - Aug 1990
Externally publishedYes

Keywords

  • Circumsporozoite (CS) protein
  • Cytololytic T cells (CTL)
  • Malaria
  • Plasmodium berghei
  • Plasmodium yoelii

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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