Isolation and characterization of protective cytolytic T cells in a rodent malaria model system

Pedro Romero; Janet L. Maryanski; Anne Sophie Cordey; Giampietro Corradin; Ruth S. Nussenzweig; Fidel Zavala

doi:10.1016/0165-2478(90)90086-6

Isolation and characterization of protective cytolytic T cells in a rodent malaria model system

Pedro Romero, Janet L. Maryanski, Anne Sophie Cordey, Giampietro Corradin, Ruth S. Nussenzweig, Fidel Zavala

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Protective immunity against malaria is induced by immunization with irradiation-attenuated sporozoites. Here we report the isolation of cytolytic T-cell (CTL) clones from BALB/c (H-2^d) mice immunized with either Plasmodium berghei or Plasmodium yoelii sporozoites. The epitopes recognized by these CTL can be mimicked by synthetic peptides corresponding to a homologous region in the CS proteins of both rodent malaria species. Both peptides are recognized by the CTL in the context of the same MHC class I molecule, H-2 K^d. In vivo adoptive transfer of the CTL clones into non-immune syngeneic mice protected them from a lethal challenge of infectious sporozoites.

Original language	English (US)
Pages (from-to)	27-31
Number of pages	5
Journal	Immunology Letters
Volume	25
Issue number	1-3
DOIs	https://doi.org/10.1016/0165-2478(90)90086-6
State	Published - Aug 1990
Externally published	Yes

Keywords

Circumsporozoite (CS) protein
Cytololytic T cells (CTL)
Malaria
Plasmodium berghei
Plasmodium yoelii

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0165-2478(90)90086-6

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title = "Isolation and characterization of protective cytolytic T cells in a rodent malaria model system",

abstract = "Protective immunity against malaria is induced by immunization with irradiation-attenuated sporozoites. Here we report the isolation of cytolytic T-cell (CTL) clones from BALB/c (H-2d) mice immunized with either Plasmodium berghei or Plasmodium yoelii sporozoites. The epitopes recognized by these CTL can be mimicked by synthetic peptides corresponding to a homologous region in the CS proteins of both rodent malaria species. Both peptides are recognized by the CTL in the context of the same MHC class I molecule, H-2 Kd. In vivo adoptive transfer of the CTL clones into non-immune syngeneic mice protected them from a lethal challenge of infectious sporozoites.",

keywords = "Circumsporozoite (CS) protein, Cytololytic T cells (CTL), Malaria, Plasmodium berghei, Plasmodium yoelii",

author = "Pedro Romero and Maryanski, {Janet L.} and Cordey, {Anne Sophie} and Giampietro Corradin and Nussenzweig, {Ruth S.} and Fidel Zavala",

note = "Funding Information: We would like to thank R. Altszuler, H. Wang, and K. Mtihlethaler for superb technical assistance and A. Zoppi for typing the manuscript. The recombinant human IL-2 was a gift from Hoffmann-La Roche. RR. was supported by a grant from Hoffmann-La Roche. This work was supported in part by the United States Agency for International Development, the Joint Rockefeller Foundation, UNDP/WHO/World Bank Special Programme for Research and Training in Tropical Diseases, the NIH, the MacArthur Foundation and Italian Ente Nazionale Idrocarburi.",

year = "1990",

month = aug,

doi = "10.1016/0165-2478(90)90086-6",

language = "English (US)",

volume = "25",

pages = "27--31",

journal = "Immunology Letters",

issn = "0165-2478",

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number = "1-3",

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T1 - Isolation and characterization of protective cytolytic T cells in a rodent malaria model system

AU - Romero, Pedro

AU - Maryanski, Janet L.

AU - Cordey, Anne Sophie

AU - Corradin, Giampietro

AU - Nussenzweig, Ruth S.

AU - Zavala, Fidel

N1 - Funding Information: We would like to thank R. Altszuler, H. Wang, and K. Mtihlethaler for superb technical assistance and A. Zoppi for typing the manuscript. The recombinant human IL-2 was a gift from Hoffmann-La Roche. RR. was supported by a grant from Hoffmann-La Roche. This work was supported in part by the United States Agency for International Development, the Joint Rockefeller Foundation, UNDP/WHO/World Bank Special Programme for Research and Training in Tropical Diseases, the NIH, the MacArthur Foundation and Italian Ente Nazionale Idrocarburi.

PY - 1990/8

Y1 - 1990/8

N2 - Protective immunity against malaria is induced by immunization with irradiation-attenuated sporozoites. Here we report the isolation of cytolytic T-cell (CTL) clones from BALB/c (H-2d) mice immunized with either Plasmodium berghei or Plasmodium yoelii sporozoites. The epitopes recognized by these CTL can be mimicked by synthetic peptides corresponding to a homologous region in the CS proteins of both rodent malaria species. Both peptides are recognized by the CTL in the context of the same MHC class I molecule, H-2 Kd. In vivo adoptive transfer of the CTL clones into non-immune syngeneic mice protected them from a lethal challenge of infectious sporozoites.

AB - Protective immunity against malaria is induced by immunization with irradiation-attenuated sporozoites. Here we report the isolation of cytolytic T-cell (CTL) clones from BALB/c (H-2d) mice immunized with either Plasmodium berghei or Plasmodium yoelii sporozoites. The epitopes recognized by these CTL can be mimicked by synthetic peptides corresponding to a homologous region in the CS proteins of both rodent malaria species. Both peptides are recognized by the CTL in the context of the same MHC class I molecule, H-2 Kd. In vivo adoptive transfer of the CTL clones into non-immune syngeneic mice protected them from a lethal challenge of infectious sporozoites.

KW - Circumsporozoite (CS) protein

KW - Cytololytic T cells (CTL)

KW - Malaria

KW - Plasmodium berghei

KW - Plasmodium yoelii

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ER -

Isolation and characterization of protective cytolytic T cells in a rodent malaria model system

Abstract

Keywords

ASJC Scopus subject areas

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