Isolation and characterization of neural stem cells from human fetal striatum

Xiaoxia Li, Jinchong Xu, Yun Bai, Xuan Wang, Xin Dai, Yinan Liu, Jun Zhang, Junhua Zou, Li Shen, Lingsong Li

Research output: Contribution to journalArticlepeer-review

Abstract

This paper described that neural stem cells (hsNSCs) were isolated and expanded rapidly from human fetal striatum in adherent culture. The population was serum- and growth factor-dependent and expressed neural stem cell markers. They were capable of multi-differentiation into neurons, astrocytes, and oligodendrocytes. When plated in the dopaminergic neuron inducing medium, human striatum neural stem cells could differentiate into tyrosine hydroxylase positive neurons. hsNSCs were morphologically homogeneous and possessed high proliferation ability. The population doubled every 44.28 h and until now it has divided for more than 82 generations in vitro. Normal human diploid karyotype was unchanged throughout the in vitro culture period. Together, this study has exploited a method for continuous and rapid expansion of human neural stem cells as pure population, which maintained the capacity to generate almost fifty percent neurons. The availability of such cells may hold great interest for basic and applied neuroscience.

Original languageEnglish (US)
Pages (from-to)425-434
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume326
Issue number2
DOIs
StatePublished - Jan 14 2005
Externally publishedYes

Keywords

  • Adherent
  • Differentiation
  • Expansion
  • Neural stem cells
  • Proliferation
  • Striatum

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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