Isolation and characterization of GFAP-positive porcine neural stem/progenitor cells derived from a GFAP-CreER T2 transgenic piglet

Eunhye Kim, Seon Ung Hwang, Junchul David Yoon, Hyunggee Kim, Gabsang Lee, Sang Hwan Hyun

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The porcine brain is gyrencephalic with similar gray and white matter composition and size more comparable to the human rather than the rodent brain; however, there is lack of information about neural progenitor cells derived from this model. Results: Here, we isolated GFAP-positive porcine neural stem cells (NSCs) from the brain explant of a transgenic piglet, with expression of CreER T2 under the control of the GFAP promoter (pGFAP-CreER T2 ). The isolated pGFAP-CreER T2 NSCs showed self-renewal and expression of representative NSC markers such as Nestin and Sox2. Pharmacological inhibition studies revealed that Notch1 signaling is necessary to maintain NSC identity, whereas serum treatment induced cell differentiation into reactive astrocytes and neurons. Conclusions: Collectively, these results indicate that GFAP promoter-driven porcine CreER T2 NSCs would be a useful tool to study neurogenesis of the porcine adult central nervous system and furthers our understanding of its potential clinical application in the future.

Original languageEnglish (US)
Article number331
JournalBMC veterinary research
Volume14
Issue number1
DOIs
StatePublished - Nov 7 2018

Keywords

  • Neural stem cells
  • Notch signaling
  • Pig
  • Reactive astrocytes

ASJC Scopus subject areas

  • veterinary(all)

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