Isolation and characterization of extracellular vesicles in stem cell-related studies

Zezhou Zhao, Dillon C. Muth, Vasiliki Mahairaki, Linzhao Cheng, Kenneth W. Witwer

Research output: ResearchChapter

Abstract

Extracellular vesicles (EVs) are small derivatives of the cell that may mediate at least in part the effects of stem cells in vitro and in vivo. In light of strong interest in stem cell-based therapies, EVs are particularly attractive as therapeutic entities because they do not replicate and may have other safety advantages over whole stem cells. Here, we examine the current literature on stem cell-derived EVs, finding that differential centrifugation remains the most widely used central purification method for EVs. We then present a common variation of ultracentrifugation-based EV enrichment, followed by a protocol for determination of particle size and concentration by single particle tracking. Notes are provided to introduce technical variations and important considerations. Since rapid shifts in technique usage are expected to occur over the next several years, we briefly review new approaches to isolation and characterization.

LanguageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages205-223
Number of pages19
Volume126
DOIs
StatePublished - 2017

Publication series

NameNeuromethods
Volume126
ISSN (Print)08932336
ISSN (Electronic)19406045

Fingerprint

Stem Cells
Extracellular Vesicles
Stem cells
Centrifugation
Purification
Particle size
Derivatives
Ultracentrifugation
Cell- and Tissue-Based Therapy
Particle Size
Safety
Therapeutics
In Vitro Techniques

Keywords

  • Exosome
  • Extracellular vesicle
  • Mesenchymal stromal cell
  • Microvesicle
  • Nanoparticle tracking analysis
  • Standardization
  • Stem cell
  • Ultracentrifugation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

Cite this

Zhao, Z., Muth, D. C., Mahairaki, V., Cheng, L., & Witwer, K. W. (2017). Isolation and characterization of extracellular vesicles in stem cell-related studies. In Neuromethods (Vol. 126, pp. 205-223). (Neuromethods; Vol. 126). Humana Press Inc.. DOI: 10.1007/978-1-4939-7024-7_14

Isolation and characterization of extracellular vesicles in stem cell-related studies. / Zhao, Zezhou; Muth, Dillon C.; Mahairaki, Vasiliki; Cheng, Linzhao; Witwer, Kenneth W.

Neuromethods. Vol. 126 Humana Press Inc., 2017. p. 205-223 (Neuromethods; Vol. 126).

Research output: ResearchChapter

Zhao, Z, Muth, DC, Mahairaki, V, Cheng, L & Witwer, KW 2017, Isolation and characterization of extracellular vesicles in stem cell-related studies. in Neuromethods. vol. 126, Neuromethods, vol. 126, Humana Press Inc., pp. 205-223. DOI: 10.1007/978-1-4939-7024-7_14
Zhao Z, Muth DC, Mahairaki V, Cheng L, Witwer KW. Isolation and characterization of extracellular vesicles in stem cell-related studies. In Neuromethods. Vol. 126. Humana Press Inc.2017. p. 205-223. (Neuromethods). Available from, DOI: 10.1007/978-1-4939-7024-7_14
Zhao, Zezhou ; Muth, Dillon C. ; Mahairaki, Vasiliki ; Cheng, Linzhao ; Witwer, Kenneth W./ Isolation and characterization of extracellular vesicles in stem cell-related studies. Neuromethods. Vol. 126 Humana Press Inc., 2017. pp. 205-223 (Neuromethods).
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