TY - JOUR
T1 - Isolation and characterization of cloned human DNA fragments carrying reiterated sequences common to both autosomes and the X chromosome
AU - Schmeckpeper, Barbara J.
AU - Willard, Huntington F.
AU - Smith, Kirby D.
N1 - Funding Information:
ACKNOWLEDGMENTS We thank Drs. T. Maniatis for the human library and AH8G1; F.H. Ruddle, B.P. Dorman and S.J. Goss for cell lines; D. George for DNA from certain hybrid cell lines; S.H. Boyer for discussion and comments during the course of the work; and D. George and K. Feden for communicating their results prior to publication. We thank V.E. Powers for karyotypic analysis of cell lines, C.C. Talbot, Jr. for assistance with the nick translations, and N. Fortunato and B. Wright for typing the manuscript. This work was supported by grants from the USPHS, NIH General Research Support Grant, RR-5378 (BJS); NIH Grant, HL15026 (KDS); and NIH Post-doctoral Training Grant, GM07471 (HFW).
PY - 1981/4/24
Y1 - 1981/4/24
N2 - Several recombinants were identified and purified from a cloned library of human DNA by virtue of their homology to DNA from a mouse-human hybrid cell line containing a single human chromosome, the X, and their lack of homology to mouse DNA. Three recombinants were characterized in detail, and all were homologous to reiterated DNA from the human X chromosome. These recombinants also were homologous to reiterated sequences on one or more human autosomes and, therefore, were not X chromosome specific. The recombinant DNA fragments homologous to human reiterated X DNA were the same fragments homologous to human reiterated autosomal DNA. Digestion of genomic DNAs with several restriction enzymes revealed that the pattern of fragments homologous to one recombinant, λHb2, was the same on autosomes as on the X chromosome, suggesting that the molecular organization of these elements on the X is not distinct from their organization on autosomes.
AB - Several recombinants were identified and purified from a cloned library of human DNA by virtue of their homology to DNA from a mouse-human hybrid cell line containing a single human chromosome, the X, and their lack of homology to mouse DNA. Three recombinants were characterized in detail, and all were homologous to reiterated DNA from the human X chromosome. These recombinants also were homologous to reiterated sequences on one or more human autosomes and, therefore, were not X chromosome specific. The recombinant DNA fragments homologous to human reiterated X DNA were the same fragments homologous to human reiterated autosomal DNA. Digestion of genomic DNAs with several restriction enzymes revealed that the pattern of fragments homologous to one recombinant, λHb2, was the same on autosomes as on the X chromosome, suggesting that the molecular organization of these elements on the X is not distinct from their organization on autosomes.
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U2 - 10.1093/nar/9.8.1853
DO - 10.1093/nar/9.8.1853
M3 - Article
C2 - 6264397
AN - SCOPUS:0019887303
SN - 0305-1048
VL - 9
SP - 1853
EP - 1872
JO - Nucleic acids research
JF - Nucleic acids research
IS - 8
ER -