Isolation and characterization of circulating tumor cells in patients with metastatic colorectal cancer

Steven J. Cohen, R. Katherine Alpaugh, Steve Gross, Shawn M. O'Hara, Denis A. Smirnov, Leon W.M.M. Terstappen, W. Jeffrey Allard, Maryann Bilbee, Jonathan D. Cheng, John P. Hoffman, Nancy L. Lewis, Ann Pellegrino, André Rogatko, Elin Sigurdson, Hao Wang, James C. Watson, Louis M. Weiner, Neal J. Meropol

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: Development of targeted therapeutic agents in colorectal cancer (CRC) is impeded by the lack of a noninvasive surrogate of drug effect. This pilot study evaluated the ability of immunomagnetic separation to isolate circulating tumor cells (CTCs) and of the fluorescent microscope system and flow cytometry to enumerate and characterize CTCs from patients with metastatic CRC. Patients and Methods: Fifty patients with metastatic CRC contributed 50 mL of blood at treatment initiation and disease evaluation timepoints. Fresh tumor specimens were obtained from 17 patients for comparison of circulating and in situ tumor cell characteristics. Epitheliall cells were magnetically isolated from whole blood targeting the antiepitheiial cell adhesion molecule (EpCAM). Circulating tumor cells were defined as EpCAM isolated, cytokeratin positive, nuclear stain positive, and CD45 negative. Total RNA was isolated from EpCAM-enriched CTCs and multigene reverse-transcriptase polymerase chain reaction analyses were performed. Results: The median number of CTCs detected by flow cytometry was 2/7.5 mL blood. Mean change in cell count was significantly different for patients with tumor progression versus nonprogression (+6.7 vs. +0.2/7.5 mL; P = 0.001). A correlation was noted between mean fluorescence intensity (flow cytometry) of cytokeratin in CTC and matched tumor specimens (r = 0.79, P = 0.06). Nearly 80% (15 of 19) of samples with ≥ 2 CTCs expressed ≥ 1 epithelial marker gene (CK19, CK20, carcinoembryonic antigen, or epidermal growth factor receptor). Conclusion: Isolating and characterizing CTCs from patients with metastatic CRC is feasible. Change in the CTC number might reflect clinical status, and flow cytometric and gene expression data suggest similarity of circulating and in situ tumor cells. Further evaluation of CTCs for pharmacodynamic and clinical monitoring in patients with CRC is warranted.

Original languageEnglish (US)
Pages (from-to)125-132
Number of pages8
JournalClinical colorectal cancer
Volume6
Issue number2
DOIs
StatePublished - Jul 2006
Externally publishedYes

Keywords

  • Cytotoxic chemotherapy
  • Surrogate tumor markers
  • Targeted therapeutics

ASJC Scopus subject areas

  • Oncology
  • Gastroenterology

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