Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering

Aaron James, Xinli Zhang, Mihaela Crisan, Winters R. Hardy, Pei Liang, Carolyn A. Meyers, Sonja Lobo, Venu Lagishetty, Martin K. Childers, Greg Asatrian, Catherine Ding, Yu Hsin Yen, Erin Zou, Kang Ting, Bruno Peault, Chia Soo

Research output: Contribution to journalArticle

Abstract

For over 15 years, human subcutaneous adipose tissue has been recognized as a rich source of tissue resident mesenchymal stem/stromal cells (MSC). The isolation of perivascular progenitor cells from human adipose tissue by a cell sorting strategy was first published in 2008. Since this time, the interest in using pericytes and related perivascular stem/stromal cell (PSC) populations fortissue engineering has significantly increased. Here, we describe a set of experiments identifying, isolating and characterizing PSC from canine tissue (N = 12 canine adipose tissue samples). Results showed that the same antibodies used for human PSC identification and isolation are cross-reactive with canine tissue (CD45, CD146, CD34). Like their human correlate, canine PSC demonstrate characteristics of MSC including cell surface marker expression, colony forming unit-fibroblast (CFU-F) inclusion, and osteogenic differentiation potential. As well, canine PSC respond to osteoinductive signals in a similar fashion as do human PSC, such as the secreted differentiation factor NEL-Like Molecule-1 (NELL-1). Nevertheless, important differences exist between human and canine PSC, including differences in baseline osteogenic potential. In summary, canine PSC represent a multipotent mesenchymogenic cell source for future translational efforts in tissue engineering.

Original languageEnglish (US)
Article numbere0177308
JournalPLoS One
Volume12
Issue number5
DOIs
StatePublished - May 1 2017

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tissue engineering
stromal cells
Tissue Engineering
Stromal Cells
Stem cells
Tissue engineering
stem cells
Canidae
Bone
Stem Cells
bones
Bone and Bones
dogs
Tissue
Mesenchymal Stromal Cells
adipose tissue
Adipose Tissue
Pericytes
Cell Separation
Subcutaneous Fat

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

James, A., Zhang, X., Crisan, M., Hardy, W. R., Liang, P., Meyers, C. A., ... Soo, C. (2017). Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering. PLoS One, 12(5), [e0177308]. https://doi.org/10.1371/journal.pone.0177308

Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering. / James, Aaron; Zhang, Xinli; Crisan, Mihaela; Hardy, Winters R.; Liang, Pei; Meyers, Carolyn A.; Lobo, Sonja; Lagishetty, Venu; Childers, Martin K.; Asatrian, Greg; Ding, Catherine; Yen, Yu Hsin; Zou, Erin; Ting, Kang; Peault, Bruno; Soo, Chia.

In: PLoS One, Vol. 12, No. 5, e0177308, 01.05.2017.

Research output: Contribution to journalArticle

James, A, Zhang, X, Crisan, M, Hardy, WR, Liang, P, Meyers, CA, Lobo, S, Lagishetty, V, Childers, MK, Asatrian, G, Ding, C, Yen, YH, Zou, E, Ting, K, Peault, B & Soo, C 2017, 'Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering', PLoS One, vol. 12, no. 5, e0177308. https://doi.org/10.1371/journal.pone.0177308
James, Aaron ; Zhang, Xinli ; Crisan, Mihaela ; Hardy, Winters R. ; Liang, Pei ; Meyers, Carolyn A. ; Lobo, Sonja ; Lagishetty, Venu ; Childers, Martin K. ; Asatrian, Greg ; Ding, Catherine ; Yen, Yu Hsin ; Zou, Erin ; Ting, Kang ; Peault, Bruno ; Soo, Chia. / Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering. In: PLoS One. 2017 ; Vol. 12, No. 5.
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