Isolation and characterization of autoreactive T cells in experimental autoimmune encephalomyelitis of the mouse

Stefan Brocke, Laura Quigley, Henry F. McFarland, Lawrence Steinman

Research output: Contribution to journalArticle

Abstract

Experimental autoimmune encephalomyelitis (EAE) can be adoptively transferred by T cells that are specific for autoantigens of the central nervous system. A variety of autoantigens and their derived peptides have been shown to be excellent stimulators for encephalitogenic T-cell lines and clones. This article describes protocols for the establishment and characterization of autoreactive T-cell lines and clones and for the study of EAE induced by these cells. The rationale for using transferred EAE models is discussed, together with advantages and disadvantages of using in vitro cultured T cells compared with Freund's adjuvant-based immunization for the induction of EAE.

Original languageEnglish (US)
Pages (from-to)458-462
Number of pages5
JournalMethods in Enzymology
Volume9
Issue number3
DOIs
StatePublished - Jun 1996
Externally publishedYes

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T-cells
Autoimmune Experimental Encephalomyelitis
T-Lymphocytes
Autoantigens
Clone Cells
Immunization
Cell Line
Freund's Adjuvant
Neurology
Cultured Cells
Central Nervous System
Peptides

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Isolation and characterization of autoreactive T cells in experimental autoimmune encephalomyelitis of the mouse. / Brocke, Stefan; Quigley, Laura; McFarland, Henry F.; Steinman, Lawrence.

In: Methods in Enzymology, Vol. 9, No. 3, 06.1996, p. 458-462.

Research output: Contribution to journalArticle

Brocke, Stefan ; Quigley, Laura ; McFarland, Henry F. ; Steinman, Lawrence. / Isolation and characterization of autoreactive T cells in experimental autoimmune encephalomyelitis of the mouse. In: Methods in Enzymology. 1996 ; Vol. 9, No. 3. pp. 458-462.
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