Isolation and characterization of autoreactive T cells in experimental autoimmune encephalomyelitis of the mouse

Stefan Brocke, Laura Quigley, Henry F. McFarland, Lawrence Steinman

Research output: Contribution to journalArticlepeer-review

Abstract

Experimental autoimmune encephalomyelitis (EAE) can be adoptively transferred by T cells that are specific for autoantigens of the central nervous system. A variety of autoantigens and their derived peptides have been shown to be excellent stimulators for encephalitogenic T-cell lines and clones. This article describes protocols for the establishment and characterization of autoreactive T-cell lines and clones and for the study of EAE induced by these cells. The rationale for using transferred EAE models is discussed, together with advantages and disadvantages of using in vitro cultured T cells compared with Freund's adjuvant-based immunization for the induction of EAE.

Original languageEnglish (US)
Pages (from-to)458-462
Number of pages5
JournalMethods in Enzymology
Volume9
Issue number3
DOIs
StatePublished - Jun 1996
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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