Isolation and characterization of a novel ligand-dependent thyroid hormone receptor-coactivating protein

Tsuyoshi Monden, Fredric E. Wondisford, Anthony N. Hollenberg

Research output: Contribution to journalArticlepeer-review

Abstract

The thyroid hormone receptor (TR) regulates the expression of target genes upon binding to triiodothyronine (T3) response elements. In the presence of T3, the TR recruits coactivating proteins that both modulate and integrate the ligand response. We report here the cloning of a novel protein using the TR ligand-binding domain as bait in the yeast two-hybrid system. Analysis of a putative full-length clone demonstrates a cDNA sequence that encodes a protein of 920 amino acids with a size of 120 kDa (p120). Alignment with known sequences shows homology to a previously identified protein of unknown function, termed skeletal muscle abundant protein. Interaction studies demonstrate that p120 interacts with the TR AF-2 domain in the presence of ligand through a 111-amino acid region, Northern analysis demonstrates widespread expression in human tissues. Cotransfection assays in CV-1 cells demonstrate that p120 enhances TR-mediated transactivation on multiple T3 response elements in the presence of T3. In addition, CREB- binding protein synergizes with p120 to enhance this effect. When linked to the GAL4 DNA-binding domain, p120 is an activator of transcription alone. Thus, p120 satisfies a number of important criteria as a nuclear receptor coactivator.

Original languageEnglish (US)
Pages (from-to)29834-29841
Number of pages8
JournalJournal of Biological Chemistry
Volume272
Issue number47
DOIs
StatePublished - Nov 21 1997

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Isolation and characterization of a novel ligand-dependent thyroid hormone receptor-coactivating protein'. Together they form a unique fingerprint.

Cite this