Isolation and characterization of a mouse gastrin cDNA clone that expresses mast-cell growth-factor activity in monkey cells

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Abstract

A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. Cloning was achieved using the pcD vector that permits expression of cDNA inserts in mammalian cells. The DNA sequence codes for a polypeptide of 166 amino acid residues including a putative signal peptide. The supernatant fluid obtained after transfection of COS-7 monkey cells with the pcD-MCGF plasmid had mouse MCGF activity. The MCGF amino acid sequence between amino acids 33 and 41, deduced from the nucleotide sequence of its cDNA, is identical to the NH2-terminal sequence of the mouse lymphokine, interleukin 3, suggesting that MCGF is related to interleukin 3.

Original languageEnglish (US)
Pages (from-to)1070-1074
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number4 I
DOIs
Publication statusPublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • General

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