Ischemic preconditioning upregulates inducible nitric oxide synthase in cardiac myocytes

Yang Wang, Yiru Guo, Shelley X. Zhang, Wen Jian Wu, Jianxun Wang, Weike Bao, Roberto Bolli

Research output: Contribution to journalArticle

Abstract

Recent evidence has shown that the cardioprotection afforded by the late phase of ischemic preconditioning (PC) is mediated by upregulation of inducible nitric oxide synthase (iNOS). However, the specific cardiac cell type(s) that express(es) iNOS in response to ischemic PC remains unknown. Thus, mice underwent a sequence of six cycles of 4-min coronary occlusion/4-min reperfusion, which induces late PC, and tissue samples were collected at serial times for measurement of mRNA (Northern) and protein levels (Western). In addition, whole heart samples were cryosectioned for in situ hybridization and immunohistochemistry. The steady-state levels of iNOS mRNA in the ischemic regions started to increase at 1 h after ischemic PC, peaked at 3 h (201 ± 31% of sham, n=5: P

Original languageEnglish (US)
Pages (from-to)5-15
Number of pages11
JournalJournal of Molecular and Cellular Cardiology
Volume34
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Ischemic Preconditioning
Nitric Oxide Synthase Type II
Cardiac Myocytes
Up-Regulation
Messenger RNA
Coronary Occlusion
Reperfusion
In Situ Hybridization
Immunohistochemistry
Proteins

Keywords

  • Gene expression
  • Infarction
  • Ischemia
  • Mouse
  • Myocardium

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Ischemic preconditioning upregulates inducible nitric oxide synthase in cardiac myocytes. / Wang, Yang; Guo, Yiru; Zhang, Shelley X.; Wu, Wen Jian; Wang, Jianxun; Bao, Weike; Bolli, Roberto.

In: Journal of Molecular and Cellular Cardiology, Vol. 34, No. 1, 2002, p. 5-15.

Research output: Contribution to journalArticle

Wang, Yang ; Guo, Yiru ; Zhang, Shelley X. ; Wu, Wen Jian ; Wang, Jianxun ; Bao, Weike ; Bolli, Roberto. / Ischemic preconditioning upregulates inducible nitric oxide synthase in cardiac myocytes. In: Journal of Molecular and Cellular Cardiology. 2002 ; Vol. 34, No. 1. pp. 5-15.
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