Is reduced SERCA2a expression detrimental or beneficial to postischemic cardiac function and injury? Evidence from heterozygous SERCA2a knockout mice

M. A Hassan Talukder, Anuradha Kalyanasundaram, Li Zuo, Murugesan Velayutham, Yoshinori Nishijima, Muthu Periasamy, Jay L. Zweier

Research output: Contribution to journalArticle

Abstract

Recent studies have demonstrated that increased expression of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) 2a improves myocardial contractility and Ca2+ handling at baseline and in disease conditions, including myocardial ischemia-reperfusion (I/R). Conversely, it has also been reported that pharmacological inhibition of SERCA might improve postischemic function in stunned hearts or in isolated myocardium following I/R. The goal of this study was to test how decreases in SERCA pump level/activity affect cardiac function following I/R. To address this question, we used a heterozygous SERCA2a knockout (SERCA2a+/-) mouse model with decreased SERCA pump levels and studied the effect of myocardial stunning (20-min ischemia followed by reperfusion) and infarction (30-min ischemia followed by reperfusion) following 60-min reperfusion. Our results demonstrate that postischemic myocardial relaxation was significantly impaired in SERCA2a +/- hearts with both stunning and infarction protocols. Interestingly, postischemic recovery of contractile function was comparable in SERCA2a+/- and wild-type hearts subjected to stunning. In contrast, following 30-min ischemia, postischemic contractile function was reduced in SERCA2a+/- hearts with significantly larger infarction. Rhod-2 spectrofluorometry revealed significantly higher diastolic intracellular Ca 2+ in SERCA2a+/- hearts compared with wild-type hearts. Both at 30-min ischemia and 2-min reperfusion, intracellular Ca2+ levels were significantly higher in SERCA2a+/- hearts. Electron paramagnetic resonance spin trapping showed a similar extent of postischemic free-radical generation in both strains. These data provide direct evidence that functional SERCA2a level, independent of oxidative stress, is crucial for postischemic myocardial function and salvage during I/R.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume294
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

Fingerprint

Knockout Mice
Reticulum
Calcium-Transporting ATPases
Wounds and Injuries
Reperfusion
Ischemia
Infarction
Myocardial Stunning
Spin Trapping
Myocardial Reperfusion
Fluorescence Spectrometry
Recovery of Function
Electron Spin Resonance Spectroscopy
Free Radicals
Myocardial Ischemia
Myocardium
Oxidative Stress
Pharmacology

Keywords

  • Calcium handling
  • Free radicals
  • Myocardial ischemia-reperfusion
  • Postischemic function
  • Sarco(endo)plasmic reticulum Ca -ATPase

ASJC Scopus subject areas

  • Physiology

Cite this

Is reduced SERCA2a expression detrimental or beneficial to postischemic cardiac function and injury? Evidence from heterozygous SERCA2a knockout mice. / Talukder, M. A Hassan; Kalyanasundaram, Anuradha; Zuo, Li; Velayutham, Murugesan; Nishijima, Yoshinori; Periasamy, Muthu; Zweier, Jay L.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 294, No. 3, 03.2008.

Research output: Contribution to journalArticle

Talukder, M. A Hassan ; Kalyanasundaram, Anuradha ; Zuo, Li ; Velayutham, Murugesan ; Nishijima, Yoshinori ; Periasamy, Muthu ; Zweier, Jay L. / Is reduced SERCA2a expression detrimental or beneficial to postischemic cardiac function and injury? Evidence from heterozygous SERCA2a knockout mice. In: American Journal of Physiology - Heart and Circulatory Physiology. 2008 ; Vol. 294, No. 3.
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