Irregular telomeres impair meiotic synapsis and recombination in mice

Lin Liu, Sonia Franco, Barbara Spyropoulos, Peter B. Moens, Maria A. Blasco, David L. Keefe

Research output: Contribution to journalArticle

Abstract

Telomere shortening can lead to chromosome instability, replicative senescence, and apoptosis in both somatic and male germ cells. To study roles for mammalian telomeres in homologous pairing and recombination, we characterized effects of telomere shortening on spermatogenesis and oogenesis in late-generation telomerase-deficient mice. We show that shortened telomeres of late-generation telomerase-deficient mice impair meiotic synapsis and decrease recombination, in particular, in females. In response to telomere shortening, male germ cells mostly undergo apoptosis, whereas female germ cells preferentially arrest in early meiosis, suggesting sexually dimorphic surveillance mechanisms for telomere dysfunction during meiosis in mice. Further, meiocytes of late-generation telomerase-deficient females with shortened telomeres, bred with early-generation males harboring relatively long telomeres, exhibit severely impaired chromosome pairing and synapsis and reduced meiotic recombination. These findings imply that functional telomeres are important in mammalian meiotic synapsis and recombination.

Original languageEnglish (US)
Pages (from-to)6496-6501
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number17
DOIs
StatePublished - Apr 27 2004
Externally publishedYes

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Telomere Shortening
Chromosome Pairing
Telomere
Genetic Recombination
Telomerase
Germ Cells
Meiosis
Apoptosis
Oogenesis
Chromosomal Instability
Cell Aging
Homologous Recombination
Spermatogenesis

Keywords

  • Meiosis
  • Mouse
  • Oocytes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Irregular telomeres impair meiotic synapsis and recombination in mice. / Liu, Lin; Franco, Sonia; Spyropoulos, Barbara; Moens, Peter B.; Blasco, Maria A.; Keefe, David L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 17, 27.04.2004, p. 6496-6501.

Research output: Contribution to journalArticle

Liu, Lin ; Franco, Sonia ; Spyropoulos, Barbara ; Moens, Peter B. ; Blasco, Maria A. ; Keefe, David L. / Irregular telomeres impair meiotic synapsis and recombination in mice. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 17. pp. 6496-6501.
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