IPMK Mediates Activation of ULK Signaling and Transcriptional Regulation of Autophagy Linked to Liver Inflammation and Regeneration

Prasun Guha, Richa Tyagi, Sayan Chowdhury, Luke Reilly, Chenglai Fu, Risheng Xu, Adam C. Resnick, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Autophagy plays a broad role in health and disease. Here, we show that inositol polyphosphate multikinase (IPMK) is a prominent physiological determinant of autophagy and is critical for liver inflammation and regeneration. Deletion of IPMK diminishes autophagy in cell lines and mouse liver. Regulation of autophagy by IPMK does not require catalytic activity. Two signaling axes, IPMK-AMPK-Sirt-1 and IPMK-AMPK-ULK1, appear to mediate the influence of IPMK on autophagy. IPMK enhances autophagy-related transcription by stimulating AMPK-dependent Sirt-1 activation, which mediates the deacetylation of histone 4 lysine 16. Furthermore, direct binding of IPMK to ULK and AMPK forms a ternary complex that facilitates AMPK-dependent ULK phosphorylation. Deletion of IPMK in cell lines and intact mice virtually abolishes lipophagy, promotes liver damage as well as inflammation, and impairs hepatocyte regeneration. Thus, targeting IPMK may afford therapeutic benefits in disabilities that depend on autophagy and lipophagy—specifically, in liver inflammation and regeneration. IPMK is a physiological determinant of autophagy and is critical in liver inflammation. Two signaling axes, IPMK-AMPK-Sirt-1 and IPMK-AMPK- ULK1, appear to mediate the influence of IPMK on autophagy. Deletion of IPMK impairs lipophagy and hepatocyte regeneration.

Original languageEnglish (US)
Pages (from-to)2692-2703.e7
JournalCell Reports
Volume26
Issue number10
DOIs
StatePublished - Mar 5 2019

Fingerprint

Liver Regeneration
Autophagy
Liver
Chemical activation
Inflammation
AMP-Activated Protein Kinases
inositol polyphosphate multikinase
Regeneration
Hepatocytes
Cells
Cell Line
Phosphorylation
Transcription
Histones
Lysine

Keywords

  • AMPK
  • autophagy
  • inositol
  • IPMK
  • lipophagy
  • liver
  • liver damage
  • regeneration
  • Sirt-1
  • ULK

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

IPMK Mediates Activation of ULK Signaling and Transcriptional Regulation of Autophagy Linked to Liver Inflammation and Regeneration. / Guha, Prasun; Tyagi, Richa; Chowdhury, Sayan; Reilly, Luke; Fu, Chenglai; Xu, Risheng; Resnick, Adam C.; Snyder, Solomon H.

In: Cell Reports, Vol. 26, No. 10, 05.03.2019, p. 2692-2703.e7.

Research output: Contribution to journalArticle

Guha, Prasun ; Tyagi, Richa ; Chowdhury, Sayan ; Reilly, Luke ; Fu, Chenglai ; Xu, Risheng ; Resnick, Adam C. ; Snyder, Solomon H. / IPMK Mediates Activation of ULK Signaling and Transcriptional Regulation of Autophagy Linked to Liver Inflammation and Regeneration. In: Cell Reports. 2019 ; Vol. 26, No. 10. pp. 2692-2703.e7.
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