Inward currents induced by ischemia in rat spinal cord dorsal horn neurons

Meng Chen, Yuan Xiang Tao, Jianguo G. Gu

Research output: Contribution to journalArticle

Abstract

Hypoxia and ischemia occur in the spinal cord when blood vessels of the spinal cord are compressed under pathological conditions such as spinal stenosis, tumors, and traumatic spinal injury. Here by using spinal cord slice preparations and patch-clamp recordings we investigated the influence of an ischemia-simulating medium on dorsal horn neurons in deep lamina, a region that plays a significant role in sensory hypersensitivity and pathological pain. We found that the ischemia-simulating medium induced large inward currents in dorsal horn neurons recorded. The onset of the ischemia-induced inward currents was age-dependent, being onset earlier in older animals. Increases of sensory input by the stimulation of afferent fibers with electrical impulses or by capsaicin significantly speeded up the onset of the ischemia-induced inward currents. The ischemia-induced inward currents were abolished by the glutamate receptor antagonists CNQX (20 μM) and APV (50 μM). The ischemia-induced inward currents were also substantially inhibited by the glutamate transporter inhibitor TBOA (100 μM). Our results suggest that ischemia caused reversal operation of glutamate transporters, leading to the release of glutamate via glutamate transporters and the subsequent activation of glutamate receptors in the spinal dorsal horn neurons.

Original languageEnglish (US)
Article number10
JournalMolecular Pain
Volume3
DOIs
StatePublished - Apr 25 2007

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Posterior Horn Cells
Ischemia
Amino Acid Transport System X-AG
Spinal Cord
6-Cyano-7-nitroquinoxaline-2,3-dione
Spinal Stenosis
Spinal Injuries
Excitatory Amino Acid Antagonists
Spinal Cord Dorsal Horn
Capsaicin
Glutamate Receptors
Fetal Blood
Blood Vessels
Glutamic Acid
Hypersensitivity
Pain

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Molecular Medicine
  • Cellular and Molecular Neuroscience

Cite this

Inward currents induced by ischemia in rat spinal cord dorsal horn neurons. / Chen, Meng; Tao, Yuan Xiang; Gu, Jianguo G.

In: Molecular Pain, Vol. 3, 10, 25.04.2007.

Research output: Contribution to journalArticle

Chen, Meng ; Tao, Yuan Xiang ; Gu, Jianguo G. / Inward currents induced by ischemia in rat spinal cord dorsal horn neurons. In: Molecular Pain. 2007 ; Vol. 3.
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