Involvement of poly ADP ribosyl polymerase-1 in acute but not chronic zinc toxicity

Christian T. Sheline, Hongmin Wang, Ai Li Cai, Valina L. Dawson, Dennis W. Choi

Research output: Contribution to journalArticle

Abstract

We have previously suggested that zinc-induced neuronal death may be mediated in part by inhibition of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH), secondary to depletion of the essential cosubstrate NAD+. Given convergent evidence implicating the NAD+-catabolizing enzyme, poly ADP ribosyl polymerase (PARP) in mediating ATP depletion and neuronal death after excitotoxic and ischemic insults, we tested the specific hypothesis that the neuronal death induced by exposure to toxic levels of extracellular zinc might be partly mediated by PARP. PARP was activated in cultured mouse cortical astrocytes after a toxic acute Zn2+ exposure (350 μM Zn2+ for 15 min), but not in cortical neurons or gila after exposure to a toxic chronic Zn2+ exposure (40 μM Zn2+ for 1-4 h), an exposure sufficient to deplete NAD+ and ATP levels. Furthermore, the neurotoxicity induced by acute, but not chronic, Zn2+ exposure was reduced in mixed neuronal-glial cultures prepared from mutant mice lacking the PARP gene. These data suggest PARP activation may contribute to more fulminant forms of Zn 2+-induced neuronal death.

Original languageEnglish (US)
Pages (from-to)1402-1409
Number of pages8
JournalEuropean Journal of Neuroscience
Volume18
Issue number6
DOIs
StatePublished - Sep 1 2003

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Keywords

  • ATP
  • Astrocyte
  • Mouse
  • NAD

ASJC Scopus subject areas

  • Neuroscience(all)

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