Involvement of interleukin-18 in the pathogenesis of human eosinophilic esophagitis

Rituraj Niranjan, Priya Rajavelu, Sathisha Upparahalli Ventateshaiah, Jai Shankar Shukla, Asifa Zaidi, Siddesha Jalahalli Mariswamy, Jochen Mattner, Ilana Fortgang, Monika Kowalczyk, Luis Balart, Anshi Shukla, Anil Mishra

Research output: Contribution to journalArticlepeer-review

Abstract

IL-18 is induced in food allergy and EoE is food allergen-induced disease. Therefore, we tested the hypothesis whether IL-18 is involved in food allergen-induced EoE pathogenesis. Accordingly, we examined normal SPT + and SPT - EoE patient blood and biopsy samples for IL-18, IL-18Rα, ICAM and VCAM expression. Herein, we show increased IL-18 level is highly significant in food allergen SPT + compared to SPT. - EoE patients. We also report that IL-18Rα + cells and mRNA levels are induced in the esophageal biopsies of EoE patients and blood IL-18 levels correlate with esophageal eosinophilia (P < 0.01). Additionally, we report that the levels of esophageal eosinophil and mast cells correlate with ICAM expression in human EoE. Mechanistically, we show that IL-18 in vitro stimulates iNKT cells and endothelial cells and induce eosinophil active cytokines IL-5 and IL-13. We provide the evidence that IL-18 is critical cytokine involved in activation of iNKT cells and ICAM in promoting human EoE.

Original languageEnglish (US)
Pages (from-to)103-113
Number of pages11
JournalClinical Immunology
Volume157
Issue number2
DOIs
StatePublished - Apr 1 2015

Keywords

  • EoE
  • ICAM
  • IL-18Rα
  • INKT cells
  • Interleukin-IL-18

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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