Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain

Makiko Miyamoto, Yoshiyuki Tsuboi, Kogo Takamiya, Richard L. Huganir, Masahiro Kondo, Masamichi Shinoda, Yoshiyuki Oi, Koichi Iwata

Research output: Contribution to journalArticlepeer-review

Abstract

To clarify the involvement of GluR2 and GluR3 subunits of AMPA receptor in orofacial neuropathic pain, we studied changes in nocifensive behavior and extracellular-signal regulated kinase (ERK) phosphorylation followed by infraorbital nerve (ION)-partial transection model applied to GluR2 or GluR3 delta7 knock-in (KI) mice. In these animals, last seven amino acids of GluR2 or GluR3 subunit, the binding sites of interacting protein, are deleted in vivo. Head-withdrawal threshold to mechanical stimulation of the whisker pad skin ipsilateral to ION-partial transection was significantly reduced at 1, 3, 5, 7, 11 and 14 days after transection compared with that before transection in wild-type mice. In the GluR2 and GluR3 delta7 KI mice, the head-withdrawal threshold did not change following ION-partial transection. The number of pERK-LI cells examined in Vc and C1-C2 in wild-type mice after the non-noxious stimulation was larger than that of GluR2 and GluR3 delta7 KI mice.The present findings suggest that GluR2 and GluR3 subunits of AMPA receptor play roles in the trigeminal nerve injury-mediated enhancement of Vc and C1-C2 neuronal excitability, and hyperalgesia.

Original languageEnglish (US)
Pages (from-to)8-12
Number of pages5
JournalNeuroscience Letters
Volume491
Issue number1
DOIs
StatePublished - Mar 10 2011

Keywords

  • AMPA receptor
  • Infraorbital nerve injury
  • Neuropathic pain
  • Phosphorylation of extracellular signal-regulated kinase

ASJC Scopus subject areas

  • Neuroscience(all)

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