TY - JOUR
T1 - Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
AU - Miyamoto, Makiko
AU - Tsuboi, Yoshiyuki
AU - Takamiya, Kogo
AU - Huganir, Richard L.
AU - Kondo, Masahiro
AU - Shinoda, Masamichi
AU - Oi, Yoshiyuki
AU - Iwata, Koichi
N1 - Funding Information:
This study was supported in part by Research Grants from Sato and Uemura Funds from Nihon University School of Dentistry, and a grant from the Dental Research Center, Nihon University School of Dentistry; Nihon University multidisciplinary research grant for KI; grants from the Ministry of Education, Culture, Sports, Science, and Technology to promote multidisciplinary research project “Translational Research Network on Orofacial Neurological Disorders” at Nihon University School of Dentistry; the Japan–Canada Joint Health Research Program 167458, NIH grant ( DE04786 ), and CHIR grants ( MOP-43095 ), ( MOP-82831 ). We also thank Prof. A.M. Binshtok for commenting on this manuscript.
PY - 2011/3/10
Y1 - 2011/3/10
N2 - To clarify the involvement of GluR2 and GluR3 subunits of AMPA receptor in orofacial neuropathic pain, we studied changes in nocifensive behavior and extracellular-signal regulated kinase (ERK) phosphorylation followed by infraorbital nerve (ION)-partial transection model applied to GluR2 or GluR3 delta7 knock-in (KI) mice. In these animals, last seven amino acids of GluR2 or GluR3 subunit, the binding sites of interacting protein, are deleted in vivo. Head-withdrawal threshold to mechanical stimulation of the whisker pad skin ipsilateral to ION-partial transection was significantly reduced at 1, 3, 5, 7, 11 and 14 days after transection compared with that before transection in wild-type mice. In the GluR2 and GluR3 delta7 KI mice, the head-withdrawal threshold did not change following ION-partial transection. The number of pERK-LI cells examined in Vc and C1-C2 in wild-type mice after the non-noxious stimulation was larger than that of GluR2 and GluR3 delta7 KI mice.The present findings suggest that GluR2 and GluR3 subunits of AMPA receptor play roles in the trigeminal nerve injury-mediated enhancement of Vc and C1-C2 neuronal excitability, and hyperalgesia.
AB - To clarify the involvement of GluR2 and GluR3 subunits of AMPA receptor in orofacial neuropathic pain, we studied changes in nocifensive behavior and extracellular-signal regulated kinase (ERK) phosphorylation followed by infraorbital nerve (ION)-partial transection model applied to GluR2 or GluR3 delta7 knock-in (KI) mice. In these animals, last seven amino acids of GluR2 or GluR3 subunit, the binding sites of interacting protein, are deleted in vivo. Head-withdrawal threshold to mechanical stimulation of the whisker pad skin ipsilateral to ION-partial transection was significantly reduced at 1, 3, 5, 7, 11 and 14 days after transection compared with that before transection in wild-type mice. In the GluR2 and GluR3 delta7 KI mice, the head-withdrawal threshold did not change following ION-partial transection. The number of pERK-LI cells examined in Vc and C1-C2 in wild-type mice after the non-noxious stimulation was larger than that of GluR2 and GluR3 delta7 KI mice.The present findings suggest that GluR2 and GluR3 subunits of AMPA receptor play roles in the trigeminal nerve injury-mediated enhancement of Vc and C1-C2 neuronal excitability, and hyperalgesia.
KW - AMPA receptor
KW - Infraorbital nerve injury
KW - Neuropathic pain
KW - Phosphorylation of extracellular signal-regulated kinase
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U2 - 10.1016/j.neulet.2010.12.060
DO - 10.1016/j.neulet.2010.12.060
M3 - Article
C2 - 21215292
AN - SCOPUS:79951512153
VL - 491
SP - 8
EP - 12
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -