Involvement of aquaporins in colorectal carcinogenesis

Chulso Moon, Jean Charles Soria, Se Jin Jang, Juna Lee, Mohammad Obaidul Hoque, Mathilde Sibony, Barry Trink, Yoon Soo Chang, David Sidransky, Li Mao

Research output: Contribution to journalArticle

Abstract

Aquaporins (AQPs) are important in controlling water permeability. As AQP1 is known as a serum-responsive gene, we hypothesized that AQP expression may be involved in the development of human cancer. By reverse transcriptase- polymerase chain reaction analysis, expression of AQPs 1, 3, and 5 was found in seven colon and colorectal cancer cell lines. Western blot analysis confirmed their expression in four of these cell lines. In situ hybridization demonstrated that during colorectal carcinogenesis, the expression of AQPs 1 and 5 was induced in early-stage disease (early dysplasia) and maintained through the late stages of colon cancer development. Expression of AQPs 1 and 5 was maintained even in metastatic lesions in the liver. These findings demonstrate that the expression of several AQPs is found in tumor cells and is associated with an early stage of colorectal cancer development. These novel observations suggest that multiple AQP expression may be advantageous to tumorigenesis, which may lead to a better understanding of colorectal carcinogenesis.

Original languageEnglish (US)
Pages (from-to)6699-6703
Number of pages5
JournalOncogene
Volume22
Issue number42 REV. ISS. 4
StatePublished - Sep 29 2003

Keywords

  • Aquaporin
  • Carcinogenesis
  • Colon cancer
  • RT-PCR
  • in situ hybridization

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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  • Cite this

    Moon, C., Soria, J. C., Jang, S. J., Lee, J., Hoque, M. O., Sibony, M., Trink, B., Chang, Y. S., Sidransky, D., & Mao, L. (2003). Involvement of aquaporins in colorectal carcinogenesis. Oncogene, 22(42 REV. ISS. 4), 6699-6703.