Investigation of slow molecular dynamics using R-CODEX

Wenbo Li; Ann McDermott

doi:10.1016/j.jmr.2012.05.019

Investigation of slow molecular dynamics using R-CODEX

Wenbo Li, Ann McDermott

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A solid state NMR experiment is introduced for probing motions on the millisecond time scale, based on dephasing and refocusing ¹H- ¹³C or ¹H-¹⁵N dipolar couplings. The method is related to the previously described Centerband-Only Detection of Exchange or CODEX experiment. The use of an R-type dipolar recoupling sequence takes advantage of the strong ¹H-¹³C or ¹H- ¹⁵N dipolar coupling, while suppressing the effect of ¹H-¹H homonuclear coupling. This approach paves the way to detect both the correlation time and reorientational angle of the dynamics in fully protonated samples. The performance of this pulse sequence is demonstrated using imidazole methyl sulfonate.

Original language	English (US)
Pages (from-to)	74-80
Number of pages	7
Journal	Journal of Magnetic Resonance
Volume	222
DOIs	https://doi.org/10.1016/j.jmr.2012.05.019
State	Published - Sep 2012
Externally published	Yes

Keywords

CODEX
H homonuclear coupling
Imidazole
Millisecond dynamics
R-CODEX
R18
Reorientation angle
Solid state NMR

ASJC Scopus subject areas

Biophysics
Biochemistry
Nuclear and High Energy Physics
Condensed Matter Physics

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10.1016/j.jmr.2012.05.019

Cite this

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title = "Investigation of slow molecular dynamics using R-CODEX",

abstract = "A solid state NMR experiment is introduced for probing motions on the millisecond time scale, based on dephasing and refocusing 1H- 13C or 1H-15N dipolar couplings. The method is related to the previously described Centerband-Only Detection of Exchange or CODEX experiment. The use of an R-type dipolar recoupling sequence takes advantage of the strong 1H-13C or 1H- 15N dipolar coupling, while suppressing the effect of 1H-1H homonuclear coupling. This approach paves the way to detect both the correlation time and reorientational angle of the dynamics in fully protonated samples. The performance of this pulse sequence is demonstrated using imidazole methyl sulfonate.",

keywords = "CODEX, H homonuclear coupling, Imidazole, Millisecond dynamics, R-CODEX, R18, Reorientation angle, Solid state NMR",

author = "Wenbo Li and Ann McDermott",

note = "Funding Information: We thank Professor Kristin Kumashiro, Dr. Benjamin Wylie and Dr. Segolene Laage for critical advice. This work was supported by National Institutes of Health grant R01GM 88724. ",

year = "2012",

month = sep,

doi = "10.1016/j.jmr.2012.05.019",

language = "English (US)",

volume = "222",

pages = "74--80",

journal = "Journal of Magnetic Resonance",

issn = "1090-7807",

publisher = "Academic Press Inc.",

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T1 - Investigation of slow molecular dynamics using R-CODEX

AU - Li, Wenbo

AU - McDermott, Ann

N1 - Funding Information: We thank Professor Kristin Kumashiro, Dr. Benjamin Wylie and Dr. Segolene Laage for critical advice. This work was supported by National Institutes of Health grant R01GM 88724.

PY - 2012/9

Y1 - 2012/9

N2 - A solid state NMR experiment is introduced for probing motions on the millisecond time scale, based on dephasing and refocusing 1H- 13C or 1H-15N dipolar couplings. The method is related to the previously described Centerband-Only Detection of Exchange or CODEX experiment. The use of an R-type dipolar recoupling sequence takes advantage of the strong 1H-13C or 1H- 15N dipolar coupling, while suppressing the effect of 1H-1H homonuclear coupling. This approach paves the way to detect both the correlation time and reorientational angle of the dynamics in fully protonated samples. The performance of this pulse sequence is demonstrated using imidazole methyl sulfonate.

AB - A solid state NMR experiment is introduced for probing motions on the millisecond time scale, based on dephasing and refocusing 1H- 13C or 1H-15N dipolar couplings. The method is related to the previously described Centerband-Only Detection of Exchange or CODEX experiment. The use of an R-type dipolar recoupling sequence takes advantage of the strong 1H-13C or 1H- 15N dipolar coupling, while suppressing the effect of 1H-1H homonuclear coupling. This approach paves the way to detect both the correlation time and reorientational angle of the dynamics in fully protonated samples. The performance of this pulse sequence is demonstrated using imidazole methyl sulfonate.

KW - CODEX

KW - H homonuclear coupling

KW - Imidazole

KW - Millisecond dynamics

KW - R-CODEX

KW - R18

KW - Reorientation angle

KW - Solid state NMR

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DO - 10.1016/j.jmr.2012.05.019

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SN - 1090-7807

VL - 222

SP - 74

EP - 80

JO - Journal of Magnetic Resonance

JF - Journal of Magnetic Resonance

ER -

Investigation of slow molecular dynamics using R-CODEX

Abstract

Keywords

ASJC Scopus subject areas

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