Investigation of mammalian ovulation with an in vitro perfused rabbit ovary preparation

E. E. Wallach, K. H. Wright, Y. Hamada

Research output: Contribution to journalArticlepeer-review


An in vitro perfused rabbit ovary preparation has been developed to study local factors responsible for the ovulatory process. This preparation enables: (1) isolation of the ovary from systemic influences, (2) direct serial observations of follicle development and ovulation, (3) addition to the perfusing medium of substances that may influence ovulation acting at the level of the ovary, and (4) correlation of the times of individual follicle rupture with ovarian contractile patterns. With this model the temporal requirements for human chorionic gonadotropin in the process of ovulation have been studied. The direct effects on ovulation of prostaglandin F2α(PGF2α), the antihistamine chlorpheniramine, and calcium deprivation were also investigated. Chlorpheniramine and EDTA each led to inhibition of ovulation and depressed ovarian contractility. The use of the isolated in vitro ovary preparation permits characterization of the local requirements for ovulation.

Original languageEnglish (US)
Pages (from-to)728-738
Number of pages11
JournalAmerican journal of obstetrics and gynecology
Issue number7
StatePublished - 1978
Externally publishedYes

ASJC Scopus subject areas

  • Obstetrics and Gynecology


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