Investigation of lipid composition of dissected sentinel lymph nodes of breast cancer patients by 7T proton MR spectroscopy

Mies A. Korteweg, Wouter B. Veldhuis, Willem P Th M Mali, Suzanne C E Diepstraten, Peter R. Luijten, Maurice A A J Van Den Bosch, René M J C Eijkemans, Paul J. Van Diest, Dennis W J Klomp

Research output: Contribution to journalArticle

Abstract

Purpose: To determine lipid composition of excised healthy and metastatic sentinel lymph nodes of breast cancer patients, as lipids are a potential discriminatory marker for malignancy. Materials and Methods: Ten breast cancer patients undergoing surgical nodal staging were included. 1H-magnetic resonance spectroscopic images (MRSI) were acquired without water and lipid suppression (resolution 3.0 Ã - 3.0 Ã - 5.0 mm 3). MRSI was compared to histopathology. Six groups of lipid resonances (5.4-5.2, 4.3-4.1, 2.8, 2.3-2.0, 1.6-1.3, 0.9 ppm) were identified. The intensity ratios of the total of these resonances to this total including the water resonance and of each lipid resonance to the total of all lipid resonances were determined. For statistical analysis, a mixed model was applied after logistic transformation. The results were expressed as ratios of the median values of these lipid compositions in metastatic to benign nodes. Results: In all, 6/32 (19%) of the excised nodes contained metastases. The ratios of the lipid resonances 5.4-5.2, 4.3-4.1, 2.8, 2.3-2.0, 1.6-1.3, 0.9 ppm between metastatic vs. benign were 0.3, 1.2, 0.2, 0.2, 1.2, and 0.9, respectively. Only the ratios of signals from unsaturated fatty acids to the total lipid signal differed significantly. Conclusion: Metastatic axillary lymph nodes contained fewer unsaturated fatty acids than benign nodes. 7T 1H-MRS may be useful for detecting axillary breast cancer metastases.

Original languageEnglish (US)
Pages (from-to)387-392
Number of pages6
JournalJournal of Magnetic Resonance Imaging
Volume35
Issue number2
DOIs
StatePublished - 2012
Externally publishedYes

Fingerprint

Protons
Magnetic Resonance Spectroscopy
Breast Neoplasms
Lipids
Unsaturated Fatty Acids
Neoplasm Metastasis
Sentinel Lymph Node
Water
Lymph Nodes
Neoplasms

Keywords

  • 7T MRI
  • H-MRS
  • breast cancer
  • lipid composition
  • lymph nodes
  • unsaturated fatty acids

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Korteweg, M. A., Veldhuis, W. B., Mali, W. P. T. M., Diepstraten, S. C. E., Luijten, P. R., Van Den Bosch, M. A. A. J., ... Klomp, D. W. J. (2012). Investigation of lipid composition of dissected sentinel lymph nodes of breast cancer patients by 7T proton MR spectroscopy. Journal of Magnetic Resonance Imaging, 35(2), 387-392. https://doi.org/10.1002/jmri.22820

Investigation of lipid composition of dissected sentinel lymph nodes of breast cancer patients by 7T proton MR spectroscopy. / Korteweg, Mies A.; Veldhuis, Wouter B.; Mali, Willem P Th M; Diepstraten, Suzanne C E; Luijten, Peter R.; Van Den Bosch, Maurice A A J; Eijkemans, René M J C; Van Diest, Paul J.; Klomp, Dennis W J.

In: Journal of Magnetic Resonance Imaging, Vol. 35, No. 2, 2012, p. 387-392.

Research output: Contribution to journalArticle

Korteweg, MA, Veldhuis, WB, Mali, WPTM, Diepstraten, SCE, Luijten, PR, Van Den Bosch, MAAJ, Eijkemans, RMJC, Van Diest, PJ & Klomp, DWJ 2012, 'Investigation of lipid composition of dissected sentinel lymph nodes of breast cancer patients by 7T proton MR spectroscopy', Journal of Magnetic Resonance Imaging, vol. 35, no. 2, pp. 387-392. https://doi.org/10.1002/jmri.22820
Korteweg, Mies A. ; Veldhuis, Wouter B. ; Mali, Willem P Th M ; Diepstraten, Suzanne C E ; Luijten, Peter R. ; Van Den Bosch, Maurice A A J ; Eijkemans, René M J C ; Van Diest, Paul J. ; Klomp, Dennis W J. / Investigation of lipid composition of dissected sentinel lymph nodes of breast cancer patients by 7T proton MR spectroscopy. In: Journal of Magnetic Resonance Imaging. 2012 ; Vol. 35, No. 2. pp. 387-392.
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title = "Investigation of lipid composition of dissected sentinel lymph nodes of breast cancer patients by 7T proton MR spectroscopy",
abstract = "Purpose: To determine lipid composition of excised healthy and metastatic sentinel lymph nodes of breast cancer patients, as lipids are a potential discriminatory marker for malignancy. Materials and Methods: Ten breast cancer patients undergoing surgical nodal staging were included. 1H-magnetic resonance spectroscopic images (MRSI) were acquired without water and lipid suppression (resolution 3.0 {\~A} - 3.0 {\~A} - 5.0 mm 3). MRSI was compared to histopathology. Six groups of lipid resonances (5.4-5.2, 4.3-4.1, 2.8, 2.3-2.0, 1.6-1.3, 0.9 ppm) were identified. The intensity ratios of the total of these resonances to this total including the water resonance and of each lipid resonance to the total of all lipid resonances were determined. For statistical analysis, a mixed model was applied after logistic transformation. The results were expressed as ratios of the median values of these lipid compositions in metastatic to benign nodes. Results: In all, 6/32 (19{\%}) of the excised nodes contained metastases. The ratios of the lipid resonances 5.4-5.2, 4.3-4.1, 2.8, 2.3-2.0, 1.6-1.3, 0.9 ppm between metastatic vs. benign were 0.3, 1.2, 0.2, 0.2, 1.2, and 0.9, respectively. Only the ratios of signals from unsaturated fatty acids to the total lipid signal differed significantly. Conclusion: Metastatic axillary lymph nodes contained fewer unsaturated fatty acids than benign nodes. 7T 1H-MRS may be useful for detecting axillary breast cancer metastases.",
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T1 - Investigation of lipid composition of dissected sentinel lymph nodes of breast cancer patients by 7T proton MR spectroscopy

AU - Korteweg, Mies A.

AU - Veldhuis, Wouter B.

AU - Mali, Willem P Th M

AU - Diepstraten, Suzanne C E

AU - Luijten, Peter R.

AU - Van Den Bosch, Maurice A A J

AU - Eijkemans, René M J C

AU - Van Diest, Paul J.

AU - Klomp, Dennis W J

PY - 2012

Y1 - 2012

N2 - Purpose: To determine lipid composition of excised healthy and metastatic sentinel lymph nodes of breast cancer patients, as lipids are a potential discriminatory marker for malignancy. Materials and Methods: Ten breast cancer patients undergoing surgical nodal staging were included. 1H-magnetic resonance spectroscopic images (MRSI) were acquired without water and lipid suppression (resolution 3.0 Ã - 3.0 Ã - 5.0 mm 3). MRSI was compared to histopathology. Six groups of lipid resonances (5.4-5.2, 4.3-4.1, 2.8, 2.3-2.0, 1.6-1.3, 0.9 ppm) were identified. The intensity ratios of the total of these resonances to this total including the water resonance and of each lipid resonance to the total of all lipid resonances were determined. For statistical analysis, a mixed model was applied after logistic transformation. The results were expressed as ratios of the median values of these lipid compositions in metastatic to benign nodes. Results: In all, 6/32 (19%) of the excised nodes contained metastases. The ratios of the lipid resonances 5.4-5.2, 4.3-4.1, 2.8, 2.3-2.0, 1.6-1.3, 0.9 ppm between metastatic vs. benign were 0.3, 1.2, 0.2, 0.2, 1.2, and 0.9, respectively. Only the ratios of signals from unsaturated fatty acids to the total lipid signal differed significantly. Conclusion: Metastatic axillary lymph nodes contained fewer unsaturated fatty acids than benign nodes. 7T 1H-MRS may be useful for detecting axillary breast cancer metastases.

AB - Purpose: To determine lipid composition of excised healthy and metastatic sentinel lymph nodes of breast cancer patients, as lipids are a potential discriminatory marker for malignancy. Materials and Methods: Ten breast cancer patients undergoing surgical nodal staging were included. 1H-magnetic resonance spectroscopic images (MRSI) were acquired without water and lipid suppression (resolution 3.0 Ã - 3.0 Ã - 5.0 mm 3). MRSI was compared to histopathology. Six groups of lipid resonances (5.4-5.2, 4.3-4.1, 2.8, 2.3-2.0, 1.6-1.3, 0.9 ppm) were identified. The intensity ratios of the total of these resonances to this total including the water resonance and of each lipid resonance to the total of all lipid resonances were determined. For statistical analysis, a mixed model was applied after logistic transformation. The results were expressed as ratios of the median values of these lipid compositions in metastatic to benign nodes. Results: In all, 6/32 (19%) of the excised nodes contained metastases. The ratios of the lipid resonances 5.4-5.2, 4.3-4.1, 2.8, 2.3-2.0, 1.6-1.3, 0.9 ppm between metastatic vs. benign were 0.3, 1.2, 0.2, 0.2, 1.2, and 0.9, respectively. Only the ratios of signals from unsaturated fatty acids to the total lipid signal differed significantly. Conclusion: Metastatic axillary lymph nodes contained fewer unsaturated fatty acids than benign nodes. 7T 1H-MRS may be useful for detecting axillary breast cancer metastases.

KW - 7T MRI

KW - H-MRS

KW - breast cancer

KW - lipid composition

KW - lymph nodes

KW - unsaturated fatty acids

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