TY - JOUR
T1 - Investigation of Blood Typing Method for Seoul National University Miniature Pig
AU - Yeom, S. C.
AU - Oh, B. C.
AU - Cho, S. Y.
AU - Park, C. G.
AU - Lee, B. C.
AU - Lee, W. J.
PY - 2009/6/1
Y1 - 2009/6/1
N2 - Pig blood group antigens may be present on grafted tissue as 16 isoforms, including the major one, A substrance. Seoul National University (SNU) miniature pigs and domestic pigs were used in this study. Polymerase chain reaction (PCR) was performed for the erythrocyte antigen A (EAA) gene, and reverse transcriptase-PCR for pig A transferase fucosyl transferase (FUT) 1 and FUT-2. The hemagglutination test was performed with murine monoclonal anti-A and anti-B antibodies (mAb), and immunohistochemistry with anti-human blood antigen mAb. SNU miniature and domestic pigs showed blood groups A and O. Blood group A SNU miniature pigs expressed either EAAAA or EAAAO and either SSS or SSO; blood group O miniature pigs expressed EAAOO and SSS or SSO, and there was no Aweak. Additionally, blood group A could be divided into blood group Aclotting and blood group Anot clotting in hemagglutination tests. Pig A substance was expressed in the lung and kidney in blood group A pigs, but we could not detect pig A substance expression in the lung, kidney, and heart of blood group O pigs or the heart of blood group A pigs. In conclusion, we suggest that blood typing of SNU miniature pigs can be easily performed using immunohistochemistry, PCR, and/or RT-PCR. Molecular-based AO typing described in this study may be useful to select SNU miniature pigs bearing a specific blood group.
AB - Pig blood group antigens may be present on grafted tissue as 16 isoforms, including the major one, A substrance. Seoul National University (SNU) miniature pigs and domestic pigs were used in this study. Polymerase chain reaction (PCR) was performed for the erythrocyte antigen A (EAA) gene, and reverse transcriptase-PCR for pig A transferase fucosyl transferase (FUT) 1 and FUT-2. The hemagglutination test was performed with murine monoclonal anti-A and anti-B antibodies (mAb), and immunohistochemistry with anti-human blood antigen mAb. SNU miniature and domestic pigs showed blood groups A and O. Blood group A SNU miniature pigs expressed either EAAAA or EAAAO and either SSS or SSO; blood group O miniature pigs expressed EAAOO and SSS or SSO, and there was no Aweak. Additionally, blood group A could be divided into blood group Aclotting and blood group Anot clotting in hemagglutination tests. Pig A substance was expressed in the lung and kidney in blood group A pigs, but we could not detect pig A substance expression in the lung, kidney, and heart of blood group O pigs or the heart of blood group A pigs. In conclusion, we suggest that blood typing of SNU miniature pigs can be easily performed using immunohistochemistry, PCR, and/or RT-PCR. Molecular-based AO typing described in this study may be useful to select SNU miniature pigs bearing a specific blood group.
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U2 - 10.1016/j.transproceed.2009.01.096
DO - 10.1016/j.transproceed.2009.01.096
M3 - Article
C2 - 19545757
AN - SCOPUS:67249151117
SN - 0041-1345
VL - 41
SP - 1921
EP - 1926
JO - Transplantation proceedings
JF - Transplantation proceedings
IS - 5
ER -