Investigation of Blood Typing Method for Seoul National University Miniature Pig

S. C. Yeom, B. C. Oh, S. Y. Cho, C. G. Park, B. C. Lee, W. J. Lee

Research output: Contribution to journalArticle

Abstract

Pig blood group antigens may be present on grafted tissue as 16 isoforms, including the major one, A substrance. Seoul National University (SNU) miniature pigs and domestic pigs were used in this study. Polymerase chain reaction (PCR) was performed for the erythrocyte antigen A (EAA) gene, and reverse transcriptase-PCR for pig A transferase fucosyl transferase (FUT) 1 and FUT-2. The hemagglutination test was performed with murine monoclonal anti-A and anti-B antibodies (mAb), and immunohistochemistry with anti-human blood antigen mAb. SNU miniature and domestic pigs showed blood groups A and O. Blood group A SNU miniature pigs expressed either EAAAA or EAAAO and either SSS or SSO; blood group O miniature pigs expressed EAAOO and SSS or SSO, and there was no Aweak. Additionally, blood group A could be divided into blood group Aclotting and blood group Anot clotting in hemagglutination tests. Pig A substance was expressed in the lung and kidney in blood group A pigs, but we could not detect pig A substance expression in the lung, kidney, and heart of blood group O pigs or the heart of blood group A pigs. In conclusion, we suggest that blood typing of SNU miniature pigs can be easily performed using immunohistochemistry, PCR, and/or RT-PCR. Molecular-based AO typing described in this study may be useful to select SNU miniature pigs bearing a specific blood group.

Original languageEnglish (US)
Pages (from-to)1921-1926
Number of pages6
JournalTransplantation proceedings
Volume41
Issue number5
DOIs
StatePublished - Jun 1 2009
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Transplantation

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