Invasive versus noninvasive measurement of allergic and cholinergic airway responsiveness in mice

Thomas Glaab, Michaela Ziegert, Ralf Baelder, Regina Korolewitz, Armin Braun, Jens M. Hohlfeld, Wayne A Mitzner, Norbert Krug, Heinz G. Hoymann

Research output: Contribution to journalArticle

Abstract

Background: This study seeks to compare the ability of repeatable invasive and noninvasive lung function methods to assess allergen-specific and cholinergic airway responsiveness (AR) in intact, spontaneously breathing BALB/c mice. Methods: Using noninvasive head-out body plethysmography and the decrease in tidal midexpiratory flow (EF50), we determined early AR (EAR) to inhaled Aspergillus fumigatus antigens in conscious mice. These measurements were paralleled by invasive determination of pulmonary conductance (GL), dynamic compliance (Cdyn) and EF50 in another group of anesthetized, orotracheally intubated mice. Results: With both methods, allergic mice, sensitized and boosted with A. fumigatus, elicitedallergen-specific EAR to A. fumigatus (p <0.05 versus controls). Dose-response studies to aerosolized methacholine (MCh) were performed in the same animals 48 h later, showing that allergic mice relative to controls were distinctly more responsive (p <0.05) and revealed acute airway inflammation as evidenced from increased eosinophils and lymphocytes in bronchoalveolar lavage. Conclusions: We conclude that invasive and noninvasive pulmonary function tests are capable of detecting both allergen-specific and cholinergic AR in intact, allergic mice. The invasive determination of GL and Cdyn is superior in sensitivity, whereas the noninvasive EF50 method is particularly appropriate for quick and repeatable screening of respiratory function in large numbers of conscious mice.

Original languageEnglish (US)
Article number139
JournalRespiratory Research
Volume6
DOIs
StatePublished - Nov 25 2005

Fingerprint

Cholinergic Agents
Aspergillus fumigatus
Allergens
Lung
Plethysmography
Methacholine Chloride
Respiratory Function Tests
Bronchoalveolar Lavage
Eosinophils
Compliance
Respiration
Head
Lymphocytes
Inflammation
Antigens

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Medicine(all)

Cite this

Glaab, T., Ziegert, M., Baelder, R., Korolewitz, R., Braun, A., Hohlfeld, J. M., ... Hoymann, H. G. (2005). Invasive versus noninvasive measurement of allergic and cholinergic airway responsiveness in mice. Respiratory Research, 6, [139]. https://doi.org/10.1186/1465-9921-6-139

Invasive versus noninvasive measurement of allergic and cholinergic airway responsiveness in mice. / Glaab, Thomas; Ziegert, Michaela; Baelder, Ralf; Korolewitz, Regina; Braun, Armin; Hohlfeld, Jens M.; Mitzner, Wayne A; Krug, Norbert; Hoymann, Heinz G.

In: Respiratory Research, Vol. 6, 139, 25.11.2005.

Research output: Contribution to journalArticle

Glaab, T, Ziegert, M, Baelder, R, Korolewitz, R, Braun, A, Hohlfeld, JM, Mitzner, WA, Krug, N & Hoymann, HG 2005, 'Invasive versus noninvasive measurement of allergic and cholinergic airway responsiveness in mice', Respiratory Research, vol. 6, 139. https://doi.org/10.1186/1465-9921-6-139
Glaab, Thomas ; Ziegert, Michaela ; Baelder, Ralf ; Korolewitz, Regina ; Braun, Armin ; Hohlfeld, Jens M. ; Mitzner, Wayne A ; Krug, Norbert ; Hoymann, Heinz G. / Invasive versus noninvasive measurement of allergic and cholinergic airway responsiveness in mice. In: Respiratory Research. 2005 ; Vol. 6.
@article{5af9dd4ce44f44ab9076909429e55c97,
title = "Invasive versus noninvasive measurement of allergic and cholinergic airway responsiveness in mice",
abstract = "Background: This study seeks to compare the ability of repeatable invasive and noninvasive lung function methods to assess allergen-specific and cholinergic airway responsiveness (AR) in intact, spontaneously breathing BALB/c mice. Methods: Using noninvasive head-out body plethysmography and the decrease in tidal midexpiratory flow (EF50), we determined early AR (EAR) to inhaled Aspergillus fumigatus antigens in conscious mice. These measurements were paralleled by invasive determination of pulmonary conductance (GL), dynamic compliance (Cdyn) and EF50 in another group of anesthetized, orotracheally intubated mice. Results: With both methods, allergic mice, sensitized and boosted with A. fumigatus, elicitedallergen-specific EAR to A. fumigatus (p <0.05 versus controls). Dose-response studies to aerosolized methacholine (MCh) were performed in the same animals 48 h later, showing that allergic mice relative to controls were distinctly more responsive (p <0.05) and revealed acute airway inflammation as evidenced from increased eosinophils and lymphocytes in bronchoalveolar lavage. Conclusions: We conclude that invasive and noninvasive pulmonary function tests are capable of detecting both allergen-specific and cholinergic AR in intact, allergic mice. The invasive determination of GL and Cdyn is superior in sensitivity, whereas the noninvasive EF50 method is particularly appropriate for quick and repeatable screening of respiratory function in large numbers of conscious mice.",
author = "Thomas Glaab and Michaela Ziegert and Ralf Baelder and Regina Korolewitz and Armin Braun and Hohlfeld, {Jens M.} and Mitzner, {Wayne A} and Norbert Krug and Hoymann, {Heinz G.}",
year = "2005",
month = "11",
day = "25",
doi = "10.1186/1465-9921-6-139",
language = "English (US)",
volume = "6",
journal = "Respiratory Research",
issn = "1465-9921",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Invasive versus noninvasive measurement of allergic and cholinergic airway responsiveness in mice

AU - Glaab, Thomas

AU - Ziegert, Michaela

AU - Baelder, Ralf

AU - Korolewitz, Regina

AU - Braun, Armin

AU - Hohlfeld, Jens M.

AU - Mitzner, Wayne A

AU - Krug, Norbert

AU - Hoymann, Heinz G.

PY - 2005/11/25

Y1 - 2005/11/25

N2 - Background: This study seeks to compare the ability of repeatable invasive and noninvasive lung function methods to assess allergen-specific and cholinergic airway responsiveness (AR) in intact, spontaneously breathing BALB/c mice. Methods: Using noninvasive head-out body plethysmography and the decrease in tidal midexpiratory flow (EF50), we determined early AR (EAR) to inhaled Aspergillus fumigatus antigens in conscious mice. These measurements were paralleled by invasive determination of pulmonary conductance (GL), dynamic compliance (Cdyn) and EF50 in another group of anesthetized, orotracheally intubated mice. Results: With both methods, allergic mice, sensitized and boosted with A. fumigatus, elicitedallergen-specific EAR to A. fumigatus (p <0.05 versus controls). Dose-response studies to aerosolized methacholine (MCh) were performed in the same animals 48 h later, showing that allergic mice relative to controls were distinctly more responsive (p <0.05) and revealed acute airway inflammation as evidenced from increased eosinophils and lymphocytes in bronchoalveolar lavage. Conclusions: We conclude that invasive and noninvasive pulmonary function tests are capable of detecting both allergen-specific and cholinergic AR in intact, allergic mice. The invasive determination of GL and Cdyn is superior in sensitivity, whereas the noninvasive EF50 method is particularly appropriate for quick and repeatable screening of respiratory function in large numbers of conscious mice.

AB - Background: This study seeks to compare the ability of repeatable invasive and noninvasive lung function methods to assess allergen-specific and cholinergic airway responsiveness (AR) in intact, spontaneously breathing BALB/c mice. Methods: Using noninvasive head-out body plethysmography and the decrease in tidal midexpiratory flow (EF50), we determined early AR (EAR) to inhaled Aspergillus fumigatus antigens in conscious mice. These measurements were paralleled by invasive determination of pulmonary conductance (GL), dynamic compliance (Cdyn) and EF50 in another group of anesthetized, orotracheally intubated mice. Results: With both methods, allergic mice, sensitized and boosted with A. fumigatus, elicitedallergen-specific EAR to A. fumigatus (p <0.05 versus controls). Dose-response studies to aerosolized methacholine (MCh) were performed in the same animals 48 h later, showing that allergic mice relative to controls were distinctly more responsive (p <0.05) and revealed acute airway inflammation as evidenced from increased eosinophils and lymphocytes in bronchoalveolar lavage. Conclusions: We conclude that invasive and noninvasive pulmonary function tests are capable of detecting both allergen-specific and cholinergic AR in intact, allergic mice. The invasive determination of GL and Cdyn is superior in sensitivity, whereas the noninvasive EF50 method is particularly appropriate for quick and repeatable screening of respiratory function in large numbers of conscious mice.

UR - http://www.scopus.com/inward/record.url?scp=28344434807&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28344434807&partnerID=8YFLogxK

U2 - 10.1186/1465-9921-6-139

DO - 10.1186/1465-9921-6-139

M3 - Article

C2 - 16309547

AN - SCOPUS:28344434807

VL - 6

JO - Respiratory Research

JF - Respiratory Research

SN - 1465-9921

M1 - 139

ER -