Intronic snoRNA biosynthesis in saccharomyces cerevisiae depends on the lariat-debranching enzyme

Intron length effects and activity of a precursor snoRNA

Siew Loon Ooi, Dmitry A. Samarsky, Maurille J. Fournier, Jef D. Boeke

Research output: Contribution to journalArticle

Abstract

The eukaryotic small nucleolar RNAs (snoRNAs) are involved in processing of pre-rRNA and modification of rRNA nucleotides. Some snoRNAs are derived from mono- or polycistronic transcription units, whereas others are encoded in introns of protein genes. The present study addresses the role of the RNA lariat-debranching enzyme (Dbr1p) in the synthesis and function of intronic snoRNAs in the yeast Saccharomyces cerevisiae. Intronic snoRNA production was determined to depend on Dbr1p. Accumulation of mature intronic snoRNAs is reduced in a dbr1 mutant; instead, intronic snoRNAs are 'trapped' within host intron lariats. Interestingly, the extent of intronic snoRNA accumulation in the form of lariats in dbr1 cells varied among different intronic snoRNAs. Intronic snoRNAs encoded within shorter introns, such as U24 and snR38, accumulate more unprocessed lariat precursors than those encoded within longer introns, e.g., U18 and snR39. This correlation was corroborated by experiments conducted with model intron:U24 snoRNA constructs. These results support a splicing-dependent exonucleolytic pathway for the biosynthesis of intronic snoRNAs. Curiously, U24 in a lariat may be functional in directing methylation of ribosomal RNA.

Original languageEnglish (US)
Pages (from-to)1096-1110
Number of pages15
JournalRNA
Volume4
Issue number9
DOIs
StatePublished - Sep 1998

Fingerprint

Small Nucleolar RNA
Saccharomyces cerevisiae
Introns
lariat debranching enzyme
Inteins
Ribosomal RNA
RNA Precursors
Methylation

Keywords

  • DBR1
  • Intronic snoRNA processing
  • rRNA methylation
  • Yeast

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Intronic snoRNA biosynthesis in saccharomyces cerevisiae depends on the lariat-debranching enzyme : Intron length effects and activity of a precursor snoRNA. / Ooi, Siew Loon; Samarsky, Dmitry A.; Fournier, Maurille J.; Boeke, Jef D.

In: RNA, Vol. 4, No. 9, 09.1998, p. 1096-1110.

Research output: Contribution to journalArticle

Ooi, Siew Loon ; Samarsky, Dmitry A. ; Fournier, Maurille J. ; Boeke, Jef D. / Intronic snoRNA biosynthesis in saccharomyces cerevisiae depends on the lariat-debranching enzyme : Intron length effects and activity of a precursor snoRNA. In: RNA. 1998 ; Vol. 4, No. 9. pp. 1096-1110.
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