Introduction of negative charge on the in vivo behavior of a hemoglobin crosslinked with bis (3,5-dibromosalicyl) sebacate

M. Thomas; B. Urbaitis; Y. Yu; E. Bucci; C. Fronticelli

Introduction of negative charge on the in vivo behavior of a hemoglobin crosslinked with bis (3,5-dibromosalicyl) sebacate

M. Thomas, B. Urbaitis, Y. Yu, E. Bucci, C. Fronticelli

Research output: Contribution to journal › Conference article › peer-review

Abstract

Human hemoglobin reacted with bis (3,5-dibromosalicyl) sebacate (XLHb-DEC) forms a stable tetramer and has a plasma half time (PHT) similar to that of other XLHbs, i.e., 3.2 hours. Studies were made to determine if the addition of negative charges to XLHb-DEC would extend its PHT. No increase in PHT was observed but larger urinary excretion of the succinylated species, as compared to XLHb-DEC, suggests that the introduction of negative charges may have led to in vivo instability of the tetramer or the molecule increased the glomerular permeability.

Original language	English (US)
Pages (from-to)	A170
Journal	Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume	22
Issue number	5
State	Published - Nov 1994
Externally published	Yes
Event	Proceedings of the 11th Congress of the International Society for Artificial Cells, Blood Substitutes and Immobilization Biotechnology, (ISABI) - Boston, MA, USA Duration: Jul 24 1994 → Jul 27 1994

ASJC Scopus subject areas

Biotechnology
Biomedical Engineering

Cite this

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title = "Introduction of negative charge on the in vivo behavior of a hemoglobin crosslinked with bis (3,5-dibromosalicyl) sebacate",

abstract = "Human hemoglobin reacted with bis (3,5-dibromosalicyl) sebacate (XLHb-DEC) forms a stable tetramer and has a plasma half time (PHT) similar to that of other XLHbs, i.e., 3.2 hours. Studies were made to determine if the addition of negative charges to XLHb-DEC would extend its PHT. No increase in PHT was observed but larger urinary excretion of the succinylated species, as compared to XLHb-DEC, suggests that the introduction of negative charges may have led to in vivo instability of the tetramer or the molecule increased the glomerular permeability.",

author = "M. Thomas and B. Urbaitis and Y. Yu and E. Bucci and C. Fronticelli",

year = "1994",

month = nov,

language = "English (US)",

volume = "22",

pages = "A170",

journal = "Artificial Cells, Blood Substitutes, and Immobilization Biotechnology",

issn = "1073-1199",

publisher = "Informa Healthcare",

number = "5",

note = "Proceedings of the 11th Congress of the International Society for Artificial Cells, Blood Substitutes and Immobilization Biotechnology, (ISABI) ; Conference date: 24-07-1994 Through 27-07-1994",

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TY - JOUR

T1 - Introduction of negative charge on the in vivo behavior of a hemoglobin crosslinked with bis (3,5-dibromosalicyl) sebacate

AU - Thomas, M.

AU - Urbaitis, B.

AU - Yu, Y.

AU - Bucci, E.

AU - Fronticelli, C.

PY - 1994/11

Y1 - 1994/11

N2 - Human hemoglobin reacted with bis (3,5-dibromosalicyl) sebacate (XLHb-DEC) forms a stable tetramer and has a plasma half time (PHT) similar to that of other XLHbs, i.e., 3.2 hours. Studies were made to determine if the addition of negative charges to XLHb-DEC would extend its PHT. No increase in PHT was observed but larger urinary excretion of the succinylated species, as compared to XLHb-DEC, suggests that the introduction of negative charges may have led to in vivo instability of the tetramer or the molecule increased the glomerular permeability.

AB - Human hemoglobin reacted with bis (3,5-dibromosalicyl) sebacate (XLHb-DEC) forms a stable tetramer and has a plasma half time (PHT) similar to that of other XLHbs, i.e., 3.2 hours. Studies were made to determine if the addition of negative charges to XLHb-DEC would extend its PHT. No increase in PHT was observed but larger urinary excretion of the succinylated species, as compared to XLHb-DEC, suggests that the introduction of negative charges may have led to in vivo instability of the tetramer or the molecule increased the glomerular permeability.

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M3 - Conference article

AN - SCOPUS:0028533325

SN - 1073-1199

VL - 22

SP - A170

JO - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology

JF - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology

IS - 5

T2 - Proceedings of the 11th Congress of the International Society for Artificial Cells, Blood Substitutes and Immobilization Biotechnology, (ISABI)

Y2 - 24 July 1994 through 27 July 1994

ER -

Introduction of negative charge on the in vivo behavior of a hemoglobin crosslinked with bis (3,5-dibromosalicyl) sebacate

Abstract

ASJC Scopus subject areas

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