Introduction of negative charge on the in vivo behavior of a hemoglobin crosslinked with bis (3,5-dibromosalicyl) sebacate

M. Thomas, B. Urbaitis, Y. Yu, E. Bucci, Clara Fronticelli

Research output: Contribution to journalArticle

Abstract

Human hemoglobin reacted with bis (3,5-dibromosalicyl) sebacate (XLHb-DEC) forms a stable tetramer and has a plasma half time (PHT) similar to that of other XLHbs, i.e., 3.2 hours. Studies were made to determine if the addition of negative charges to XLHb-DEC would extend its PHT. No increase in PHT was observed but larger urinary excretion of the succinylated species, as compared to XLHb-DEC, suggests that the introduction of negative charges may have led to in vivo instability of the tetramer or the molecule increased the glomerular permeability.

Original languageEnglish (US)
JournalArtificial Cells, Blood Substitutes, and Biotechnology
Volume22
Issue number5
StatePublished - Nov 1994
Externally publishedYes

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Hemoglobin
Hemoglobins
Plasmas
Permeability
Molecules
bis(3,5-dibromosalicyl)sebacate
bis(3,5-dibromosalicyl)sebacate-crosslinked hemoglobin

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials
  • Hematology

Cite this

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T1 - Introduction of negative charge on the in vivo behavior of a hemoglobin crosslinked with bis (3,5-dibromosalicyl) sebacate

AU - Thomas, M.

AU - Urbaitis, B.

AU - Yu, Y.

AU - Bucci, E.

AU - Fronticelli, Clara

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N2 - Human hemoglobin reacted with bis (3,5-dibromosalicyl) sebacate (XLHb-DEC) forms a stable tetramer and has a plasma half time (PHT) similar to that of other XLHbs, i.e., 3.2 hours. Studies were made to determine if the addition of negative charges to XLHb-DEC would extend its PHT. No increase in PHT was observed but larger urinary excretion of the succinylated species, as compared to XLHb-DEC, suggests that the introduction of negative charges may have led to in vivo instability of the tetramer or the molecule increased the glomerular permeability.

AB - Human hemoglobin reacted with bis (3,5-dibromosalicyl) sebacate (XLHb-DEC) forms a stable tetramer and has a plasma half time (PHT) similar to that of other XLHbs, i.e., 3.2 hours. Studies were made to determine if the addition of negative charges to XLHb-DEC would extend its PHT. No increase in PHT was observed but larger urinary excretion of the succinylated species, as compared to XLHb-DEC, suggests that the introduction of negative charges may have led to in vivo instability of the tetramer or the molecule increased the glomerular permeability.

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