This unit provides a set of protocols for introducing large insert DNA into cultured mammalian cells and embryos. Two different methods, spheroplast fusion and lipofection, are described for effecting transfer of YACs or gel-purified YAC DNA into cells. Additional protocols discuss preparing and transferring BACs into cells by lipofection and into embryos by microinjection.
|Original language||English (US)|
|Pages (from-to)||Unit 5.12|
|Journal||Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]|
|State||Published - Nov 2001|
ASJC Scopus subject areas