Intrinsically disordered protein RBM14 plays a role in generation of RNA:DNA hybrids at double-strand break sites

Yumi Jang, Zeinab Elsayed, Rebeka Eki, Shuaixin He, Kang Ping Du, Tarek Abbas, Mihoko Kai

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Accumulating evidence suggests participation of RNA-binding proteins with intrinsically disordered domains (IDPs) in the DNA damage response (DDR). These IDPs form liquid compartments at DNA damage sites in a poly(ADP ribose) (PAR)-dependent manner. However, it is greatly unknown how the IDPs are involved in DDR. We have shown previously that one of the IDPs RBM14 is required for the canonical nonhomologous end joining (cNHEJ). Here we show that RBM14 is recruited to DNA damage sites in a PARP- and RNA polymerase II (RNAPII)-dependent manner. Both KU and RBM14 are required for RNAPII-dependent generation of RNA:DNA hybrids at DNA damage sites. In fact, RBM14 binds to RNA:DNA hybrids. Furthermore, RNA:DNA hybrids and RNAPII are detected at gene-coding as well as at intergenic areas when double-strand breaks (DSBs) are induced. We propose that the cNHEJ pathway utilizes damage-induced transcription and intrinsically disordered protein RBM14 for efficient repair of DSBs.

Original languageEnglish (US)
Pages (from-to)5329-5338
Number of pages10
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number10
DOIs
StatePublished - Mar 10 2020

Keywords

  • DNA repair
  • Double-strand breaks
  • RBM14

ASJC Scopus subject areas

  • General

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