Abstract
The influence of GM + IL-4 and Flt3 ligand (FL) on phenotype and function of BM-derived DC from Lewis rats was investigated. GM + IL-4-induced DC, despite expression of CD80/CD86, were less stimulatory than FL-induced DC that expressed low CD80/CD86 and were efficient stimulators of allogeneic T cells. GM + IL-4 DC were CD11b+ OX62lo, whereas FL DC were CD11blo OX62+. Following activation, GM + IL-4 DC produced IL-10 and IL-6, but no IL-12p70, and were resistant to further maturation. FL DC produced IL-12p70, IFN-α/β, IL-10 and IL-6 and underwent maturation. Repeated stimulation of T cells with GM + IL-4 DC inhibited proliferation, cytokine production and induced early T cell apoptosis. FL DC-activated T cells produced large amounts of IFN-γ/IL-10 and exhibited late T cell apoptosis/necrosis. In vivo, GM + IL-4 DC induced alloAg-specific hyporesponsiveness following T cell restimulation. These results demonstrate that GM + IL-4 DC display intrinsic regulatory properties, inducing passive-cell-death in T cells with potential for inactivation/regulation of alloreactive T cells in transplantation.
Original language | English (US) |
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Pages (from-to) | 176-189 |
Number of pages | 14 |
Journal | Clinical Immunology |
Volume | 123 |
Issue number | 2 |
DOIs | |
State | Published - May 2007 |
Externally published | Yes |
Keywords
- Bone marrow
- Cytokines
- Dendritic cells
- Hyporesponsiveness
- Passive-cell-death
- Rat
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology