Intravital investigation of the effects of serotonin and glutamate on the dynamics of DNA activity in L-RPl1 neurons of edible snail

A. V. Shevelkin, V. P. Nikitin, V. V. Sherstnev

Research output: Contribution to journalArticlepeer-review

Abstract

Microloci of varying size with increased fluorescence were observed in L-RPl1 neuronal nuclei of snails using DNA-selective dye SYTO16. Application of serotonin and glutamate increased the number of small (<1 μ in diameter) microloci, while the number of medium and large (1-3 μ) loci decreased. Combined application of neurotransmitters produced more pronounced changes in the number of microloci compared to individual administration. RNA synthesis inhibitors abolished the effects of the transmitters. We hypothesized that the revealed small microloci of fluorescence are "active" DNA zones, where transcription of new genes are initiated.

Original languageEnglish (US)
Pages (from-to)563-567
Number of pages5
JournalBulletin of Experimental Biology and Medicine
Volume148
Issue number4
DOIs
StatePublished - Oct 1 2009
Externally publishedYes

Keywords

  • DNA
  • Glutamate
  • Neuron
  • SYTO16
  • Serotonin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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