TY - JOUR
T1 - Intrauterine administration of endotoxin leads to motor deficits in a rabbit model
T2 - a link between prenatal infection and cerebral palsy
AU - Saadani-Makki, Fadoua
AU - Kannan, Sujatha
AU - Lu, Xin
AU - Janisse, James
AU - Dawe, Elizabeth
AU - Edwin, Samuel
AU - Romero, Roberto
AU - Chugani, Diane
PY - 2008/12
Y1 - 2008/12
N2 - Objective: This study was undertaken to determine whether maternal intrauterine endotoxin administration leads to neurobehavioral deficits in newborn rabbits. Study Design: Pregnant New Zealand white rabbits were injected with 1 mL saline solution (n = 8) or 20 μg/kg of lipopolysaccharide in saline solution (n = 8) into the uterine wall on day 28/31 of gestation. On postnatal day 1, kits (saline solution [n = 30] and lipolysaccharide in saline solution [n = 18] from 4 consecutive litters) underwent neurobehavioral testing. Neonatal brains were stained for microglial cells and myelin. Results: Kits in the lipopolysaccharide in saline solution group were hypertonic and demonstrated significant impairment in posture, righting reflex, locomotion, and feeding, along with neuroinflammation indicated by activated microglia and hypomyelination in the periventricular regions. A greater mortality was noted in the lipopolysaccharide in saline solution group (16 stillbirths from 3 litters vs 3 from 1 litter). Conclusion: Maternal intrauterine endotoxin administration leads to white matter injury and motor deficits in the newborn rabbit, resulting in a phenotype that resembles those found in periventricular leukomalacia and cerebral palsy.
AB - Objective: This study was undertaken to determine whether maternal intrauterine endotoxin administration leads to neurobehavioral deficits in newborn rabbits. Study Design: Pregnant New Zealand white rabbits were injected with 1 mL saline solution (n = 8) or 20 μg/kg of lipopolysaccharide in saline solution (n = 8) into the uterine wall on day 28/31 of gestation. On postnatal day 1, kits (saline solution [n = 30] and lipolysaccharide in saline solution [n = 18] from 4 consecutive litters) underwent neurobehavioral testing. Neonatal brains were stained for microglial cells and myelin. Results: Kits in the lipopolysaccharide in saline solution group were hypertonic and demonstrated significant impairment in posture, righting reflex, locomotion, and feeding, along with neuroinflammation indicated by activated microglia and hypomyelination in the periventricular regions. A greater mortality was noted in the lipopolysaccharide in saline solution group (16 stillbirths from 3 litters vs 3 from 1 litter). Conclusion: Maternal intrauterine endotoxin administration leads to white matter injury and motor deficits in the newborn rabbit, resulting in a phenotype that resembles those found in periventricular leukomalacia and cerebral palsy.
KW - cerebral palsy
KW - intrauterine inflammation
KW - microglia
KW - perinatal brain injury
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U2 - 10.1016/j.ajog.2008.06.090
DO - 10.1016/j.ajog.2008.06.090
M3 - Article
C2 - 18845289
AN - SCOPUS:57149133787
SN - 0002-9378
VL - 199
SP - 651.e1-651.e7
JO - American journal of obstetrics and gynecology
JF - American journal of obstetrics and gynecology
IS - 6
ER -